Ponce-Monter Héctor, Fernández-Martínez Eduardo, Ortiz Mario I, Ramírez-Montiel Martha L, Cruz-Elizalde Delia, Pérez-Hernández Nury, Cariño-Cortés Raquel
Área Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Medico.
J Smooth Muscle Res. 2010;46(6):309-19. doi: 10.1540/jsmr.46.309.
Aloysia triphylla is traditionally utilized for the treatment of menstrual colic (primary dysmenorrhea) in Mexico. Citral is the main chemical component found in Aloysia triphylla leaves extract. Primary dysmenorrhea is a very frequent gynecological disorder in menstruating women, affecting 30-60% of them. It is usually treated with non-steroidal anti-inflammatory drugs (NSAIDs); although their effect is rapid, they possess many side effects. Due to these shortcomings, Mexican folk therapy is considered as a feasible alternative. The effects of the hexane extract of Aloysia triphylla and citral on uterine contractions were evaluated in vitro as well as their anti-inflammatory properties and gastric wound capabilities were assessed in vivo. The inhibitory effects on the contractions were analyzed using isolated uterus strips from estrogen primed rats. Contractions were induced by KCl 60 mM, oxytocin 10 mIU/mL, charbacol 10 µM and PGF(2α) 5 µM. The anti-inflammatory effect was assessed on carrageenan-induced rat hind paw edema model. The inhibitory concentration-50 (IC(50)) of the hexane extract of Aloysia triphylla upon each contractile response was for KCl 44.73 ± 2.48 µg/mL, oxytocin 42.16 ± 3.81 µg/mL, charbacol 41.87 ± 1.73 µg/mL and PGF(2α) 28.70 ± 2.40 µg/mL in a concentration-dependent way. The extract of Aloysia triphylla produced a significant inhibitory effect on PGF(2α)-induced contraction compared to its inhibitory actions on the others. Citral exhibited the same inhibitory effect on the contraction induced by PGF(2α). The oral administration of the extract (100-800 mg/kg) and citral (100-800 mg/kg) showed anti-inflammatory activity; furthermore, the maximal dose utilized did not produce gastric injury. These results were compared with anti-inflammatory effects and gastric damage produced by 30 mg/kg of indomethacin p.o. The spasmolytic and anti-inflammatory effects support the traditional use of Aloysia triphylla leaves in the treatment of the primary dysmenorrhea in Mexican communities.
在墨西哥，传统上使用三叶轮钟草来治疗痛经（原发性痛经）。柠檬醛是三叶轮钟草叶提取物中的主要化学成分。原发性痛经是月经女性中非常常见的妇科疾病，影响30%至60%的女性。通常用非甾体抗炎药（NSAIDs）治疗；尽管其效果迅速，但有许多副作用。由于这些缺点，墨西哥民间疗法被认为是一种可行的替代方法。评估了三叶轮钟草的己烷提取物和柠檬醛对子宫收缩的体外作用，以及它们在体内的抗炎特性和胃损伤修复能力。使用来自雌激素预处理大鼠的离体子宫条分析对收缩的抑制作用。收缩由60 mM氯化钾、10 mIU/mL催产素、10 µM炭酰胆碱和5 µM前列腺素F2α诱导。在角叉菜胶诱导的大鼠后爪水肿模型上评估抗炎作用。三叶轮钟草己烷提取物对每种收缩反应的半数抑制浓度（IC50）分别为：氯化钾44.73±2.48 µg/mL、催产素42.16±3.81 µg/mL、炭酰胆碱41.87±1.73 µg/mL和前列腺素F2α 28.70±2.40 µg/mL，呈浓度依赖性。与对其他物质的抑制作用相比，三叶轮钟草提取物对前列腺素F2α诱导的收缩产生了显著的抑制作用。柠檬醛对前列腺素F2α诱导的收缩表现出相同的抑制作用。提取物（100 - 800 mg/kg）和柠檬醛（100 - 800 mg/kg）口服显示出抗炎活性；此外，所用的最大剂量未产生胃损伤。将这些结果与口服30 mg/kg吲哚美辛产生的抗炎作用和胃损伤进行了比较。解痉和抗炎作用支持了墨西哥社区传统上使用三叶轮钟草叶治疗原发性痛经。
