不同冷冻保护剂在公猪精液冷冻保存中的评估

Evaluation of different cryoprotectants (CPAs) in boar semen cryopreservation.

作者信息

Kim Suhee, Lee Young-Jun, Ji Dong-Beom, Kim Yong-Jun

机构信息

Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.

出版信息

J Vet Med Sci. 2011 Jul;73(7):961-3. doi: 10.1292/jvms.10-0345. Epub 2011 Mar 28.

DOI:10.1292/jvms.10-0345

PMID:21441721

Abstract

This study was performed to evaluate the use of dimethylacetamide (DMA) and dimethyl sulfoxide (DMSO) in boar sperm cryopreservation. Semen from eight boars was cryopreserved following treatment with 3, 5, and 7% DMA and DMSO, and 3% glycerol (control). After thawing, sperm conventional parameters and membrane integrities were evaluated. There were no significant differences among different DMA concentrations in all evaluations. Membrane intactness were higher in 5% and 7% DMSO than 3% DMSO (P<0.05). Sperm motility of 5% DMSO was lower than that of 3% glycerol (P<0.005), and membrane intactness were lower in 5% DMA and DMSO than 3% glycerol (P<0.05). DMA and DMSO didn't improve sperm quality and glycerol remains the most useful for boar sperm cryopreservation.

摘要

本研究旨在评估二甲基乙酰胺（DMA）和二甲基亚砜（DMSO）在公猪精子冷冻保存中的应用。用3%、5%和7%的DMA、DMSO以及3%甘油（对照）处理八头公猪的精液后进行冷冻保存。解冻后，评估精子的常规参数和膜完整性。在所有评估中，不同DMA浓度之间无显著差异。5%和7%DMSO组的膜完整性高于3%DMSO组（P<0.05）。5%DMSO组的精子活力低于3%甘油组（P<0.005），5%DMA和DMSO组的膜完整性低于3%甘油组（P<0.05）。DMA和DMSO并未改善精子质量，甘油仍是公猪精子冷冻保存中最有效的物质。