Santiani A, Evangelista-Vargas S, Vargas S, Gallo S, Ruiz L, Orozco V, Rosemberg M
Laboratory of Reproductive and Cellular Biotechnologies, Faculty of Veterinary and Biological Sciences, Universidad Científica del Sur, Lima, Peru.
Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru.
Andrologia. 2017 Aug;49(6). doi: 10.1111/and.12672. Epub 2016 Aug 3.
The objective was to evaluate the effect of different cryoprotectant agents in the cryopreservation of Peruvian Paso horse semen. Twenty semen samples were collected from five Peruvian Paso horse stallions. Each sample was divided into 12 parts to form the groups: dimethylacetamide (DMA), dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol (GLY), at 3%, 4% and 5%. Samples were frozen using a rate-controlled freezer. Sperm parameters evaluated were motility and viability/acrosomal status. After thawing, progressive motility in DMA group was higher (p < .05) than in DMSO, EG and GLY groups. Similarly, viable acrosome-intact spermatozoa were higher (p < .05) using DMA in comparison with DMSO. No differences were found when comparing concentrations for any of the cryoprotectant agents. In conclusion, DMA seems to be a good cryoprotectant agent for the cryopreservation of Peruvian Paso horse stallion semen.
目的是评估不同冷冻保护剂在秘鲁帕索马精液冷冻保存中的作用。从五匹秘鲁帕索种公马采集了20份精液样本。每个样本分成12份，形成3%、4%和5%浓度的二甲基乙酰胺（DMA）、二甲基亚砜（DMSO）、乙二醇（EG）和甘油（GLY）组。使用程序降温冷冻仪对样本进行冷冻。评估的精子参数为活力和存活/顶体状态。解冻后，DMA组的前向运动性高于DMSO、EG和GLY组（p < 0.05）。同样，与DMSO相比，使用DMA时存活且顶体完整的精子比例更高（p < 0.05）。比较任何一种冷冻保护剂的不同浓度时均未发现差异。总之，DMA似乎是秘鲁帕索种公马精液冷冻保存的一种良好冷冻保护剂。
