Biochemistry and Molecular Biology Laboratory, Centre of Advanced Study, Department of Zoology, Banaras Hindu University, Varanasi, 221 005, India.
Biogerontology. 2011 Aug;12(4):321-8. doi: 10.1007/s10522-011-9330-y. Epub 2011 Mar 26.
Estrogen mediates its multiple functions in the brain through the recruitment of a number of interacting proteins. In this paper, we report the identification of 160 kD interacting nuclear protein of estrogen receptor (ER)β-transactivation domain (TAD) as amplified in breast cancer 1(AIB1) by pull down assay, immunoblotting, far-western analysis and immunoprecipitation. Further we show the age dependent interaction and expression of AIB1 in the brain of young (6 weeks), adult (25 weeks) and old (70 weeks) AKR strain mice of both sexes. The immunoprecipitation data revealed higher interaction of AIB1 in young than adult and old male mice. In contrast, the interaction was low in young, increased in adult but decreased in old female. However, immunoblotting showed age related increase in the expression of AIB1 in both male and female mice. Further, the level of interaction of AIB1 with ERβTAD in young and old male was significantly higher than female of same age, whereas the expression of AIB1 in adult and old female was significantly higher than male of same age. These data suggest that such age dependent variation in the interaction of AIB1 with ERβTAD and its expression may be helpful to regulate estrogen-mediated gene functions during aging and neurodegenerative diseases.
雌激素通过招募许多相互作用的蛋白质来介导其在大脑中的多种功能。在本文中，我们通过下拉实验、免疫印迹、远 Western 分析和免疫沉淀，报告了鉴定出的与雌激素受体 (ER)β-转录激活结构域 (TAD) 相互作用的 160kD 核蛋白，即乳腺癌 1（AIB1）。此外，我们还展示了年龄依赖性的 AKR 品系雌雄小鼠大脑中 AIB1 的相互作用和表达，该品系包括幼鼠（6 周）、成年鼠（25 周）和老年鼠（70 周）。免疫沉淀数据显示，雄性幼鼠中 AIB1 的相互作用高于成年和老年鼠。相比之下，在幼鼠中相互作用较低，在成年鼠中增加，但在老年鼠中减少。然而，免疫印迹显示，无论雌雄，AIB1 的表达均随年龄增长而增加。此外，在幼鼠和老年鼠中，AIB1 与 ERβTAD 的相互作用水平明显高于同年龄的雌性，而在成年和老年雌性中，AIB1 的表达明显高于同年龄的雄性。这些数据表明，AIB1 与 ERβTAD 的相互作用及其表达的这种年龄依赖性变化可能有助于调节衰老和神经退行性疾病期间雌激素介导的基因功能。
