Department of Otorhinolaryngology, Sanggye Paik Hospital, College of Medicine, Inje University, Seoul, Korea.
Phytother Res. 2011 May;25(5):760-4. doi: 10.1002/ptr.3488. Epub 2011 Mar 28.
In this study, the effects of oleanolic acid and ursolic acid on MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA) from human airway epithelial cells were investigated. Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with EGF and PMA for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. Oleanolic acid and ursolic acid were found to inhibit the production of MUC5AC mucin protein induced by EGF and PMA, and both compounds also inhibited the expression of MUC5AC mucin gene induced by EGF and PMA. These results suggest that oleanolic acid and ursolic acid can regulate mucin gene expression, and production of mucin protein, by directly acting on airway epithelial cells.
本研究探讨了齐墩果酸和熊果酸对表皮生长因子（EGF）和佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）诱导的人气道上皮细胞 MUC5AC 粘蛋白产生和基因表达的影响。将 NCI-H292 细胞用各试剂预处理 30min，然后分别用 EGF 和 PMA 刺激 24h。通过 RT-PCR 和 ELISA 测量 MUC5AC 粘蛋白基因表达和粘蛋白蛋白的产生。发现齐墩果酸和熊果酸抑制 EGF 和 PMA 诱导的 MUC5AC 粘蛋白蛋白的产生，并且这两种化合物还抑制 EGF 和 PMA 诱导的 MUC5AC 粘蛋白基因的表达。这些结果表明，齐墩果酸和熊果酸可以通过直接作用于气道上皮细胞来调节粘蛋白基因表达和粘蛋白蛋白的产生。
