Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Daejeon, Korea.
Phytother Res. 2012 Dec;26(12):1784-8. doi: 10.1002/ptr.4650. Epub 2012 Mar 3.
In this study, we investigated whether apigenin and wogonin affect MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12-myristate 13-acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or EGF for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The results were as follows: (i) apigenin and wogonin were found to inhibit the production of MUC5AC mucin protein induced by PMA or EGF; (ii) both compounds also inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. These results suggest that apigenin and wogonin can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells.
在这项研究中，我们研究了芹菜素和白杨素是否会影响人呼吸道上皮细胞中由佛波酯（佛波醇 12-肉豆蔻酸 13-醋酸酯，PMA）或表皮生长因子（EGF）诱导的 MUC5AC 粘蛋白的产生和基因表达。用每种药物处理汇合的 NCI-H292 细胞 30min，然后分别用 PMA 或 EGF 刺激 24h。通过逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）测量 MUC5AC 粘蛋白基因表达和粘蛋白蛋白的产生。结果如下：（i）发现芹菜素和白杨素可抑制 PMA 或 EGF 诱导的 MUC5AC 粘蛋白蛋白的产生；（ii）这两种化合物还抑制了 PMA 或 EGF 诱导的 MUC5AC 粘蛋白基因的表达。这些结果表明，芹菜素和白杨素可以通过直接作用于呼吸道上皮细胞来抑制粘蛋白基因的表达和粘蛋白蛋白的产生。
