Department of Biomaterials, Göteborg University, 405 30 Göteborg, Sweden.
J Biomed Mater Res B Appl Biomater. 2011 May;97(2):364-72. doi: 10.1002/jbm.b.31823. Epub 2011 Mar 25.
The aim of the present study was to compare the early interactions between leukocytes and three different surface modifications, suggested as bioactive. Blasted titanium discs were modified by alkali and heat treatment, sodium fluoride treatment, or hydroxyapatite coating. A number of these discs were also immersed in simulated body fluid (SBF) for a week, a treatment which yielded high levels of calcium and phosphate on each surface type. The specimens were exposed for human venous blood for 32 minutes and the respiratory burst response was measured in terms of reactive oxygen species with a luminometer, and coverage of viable cells with a fluorescence microscope after staining steps. The topography, morphology, and chemistry of the surfaces were evaluated with optical interferometry and scanning electron microscopy/energy dispersive X-ray analysis (SEM/EDX). A high respiratory burst response was found for HA coated titanium in comparison with the other surface groups (p < 0.0005). The SBF immersion resulted in an increased respiratory burst response (p < 0.0005) and removed statistically significant differences between the surface groups. Thus, the results in the present study indicate that different titanium surface modifications influence the early inflammatory response differently, and that calcium phosphate compounds increase the inflammatory response.
本研究的目的是比较白细胞与三种不同表面改性之间的早期相互作用，这些改性被认为具有生物活性。经过碱热处理、氟化钠处理或羟基磷灰石涂层处理的钛盘被改性。这些圆盘的一部分也被浸入模拟体液（SBF）中一周，这一处理使每种表面类型的钙和磷水平都升高。将这些标本暴露于人体静脉血中 32 分钟，并用发光计测量活性氧的呼吸爆发反应，并在染色步骤后用荧光显微镜测量活细胞的覆盖率。用光学干涉仪和扫描电子显微镜/能量色散 X 射线分析（SEM/EDX）评估表面的形貌、形态和化学性质。与其他表面组相比，HA 涂层钛的呼吸爆发反应较高（p < 0.0005）。SBF 浸泡导致呼吸爆发反应增加（p < 0.0005），并消除了表面组之间统计学上的显著差异。因此，本研究的结果表明，不同的钛表面改性以不同的方式影响早期炎症反应，而钙磷化合物会增加炎症反应。
