Arvidsson Anna, Franke-Stenport Victoria, Andersson Martin, Kjellin Per, Sul Young-Taeg, Wennerberg Ann
Department of Biomaterials, Institute of Surgical Sciences, Göteborg University, Box 412, Goteborg 405 30, Sweden.
J Mater Sci Mater Med. 2007 Oct;18(10):1945-54. doi: 10.1007/s10856-007-3097-3. Epub 2007 Jun 7.
The aim of the present study was to compare the nucleating and growing behaviour on four types of bioactive surfaces by using the simulated body fluid (SBF) model. Titanium discs were blasted and then prepared by alkali and heat treatment, anodic oxidation, fluoridation, or hydroxyapatite coating. The discs were immersed in SBF for 1, 2, 4 and 6 weeks. Calcium phosphates were found on all specimens, as analysed with scanning electron microscopy/energy dispersive X-ray analysis (SEM/EDX). After 1 and 2 weeks of SBF immersion more titanium was accessible with SEM/EDX on the blasted surfaces than the four bioactive surface types, indicating a difference in coverage by calcium phosphates. The Ca/P mean ratio of the surfaces was approximately 1.5 after 1 week, in contrast to the fluoridated specimens which displayed a Ca/P mean ratio of approximately 2. Powder X-ray diffraction (P-XRD) analyses showed the presence of hydroxyapatite on all types of surfaces after 4 and 6 weeks of immersion. The samples immersed for 6 weeks showed a higher degree of crystallinity than the samples immersed for 4 weeks. In conclusion, differences appeared at the early SBF immersion times of 1 and 2 weeks between controls and bioactive surface types, as well as between different bioactive surface types.
本研究的目的是通过使用模拟体液（SBF）模型来比较四种生物活性表面上的成核和生长行为。对钛盘进行喷砂处理，然后通过碱热处理、阳极氧化、氟化或羟基磷灰石涂层进行制备。将这些圆盘浸入SBF中1、2、4和6周。通过扫描电子显微镜/能量色散X射线分析（SEM/EDX）分析发现，所有试样上均有磷酸钙。在SBF中浸泡1周和2周后，与四种生物活性表面类型相比，喷砂表面上通过SEM/EDX可检测到更多的钛，这表明磷酸钙的覆盖存在差异。浸泡1周后，表面的Ca/P平均比率约为1.5，而氟化试样的Ca/P平均比率约为2。粉末X射线衍射（P-XRD）分析表明，浸泡4周和6周后，所有类型的表面上均存在羟基磷灰石。浸泡6周的样品比浸泡4周的样品具有更高的结晶度。总之，在浸泡1周和2周的早期SBF浸泡时间，对照和生物活性表面类型之间以及不同生物活性表面类型之间均出现了差异。
