Universidade Federal do Rio de Janeiro, Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Rio de Janeiro, RJ, Brazil.
J Sep Sci. 2011 May;34(9):971-7. doi: 10.1002/jssc.201000901. Epub 2011 Mar 25.
In this work we report the purification of a crude acetin mixture into mono-, di- and triacetin by countercurrent chromatography. The process was initially tested on a small, semi-preparative scale (0.5 g) to determine its efficiency. The process was then scaled up to accommodate 2.5 g of crude reaction products containing a mixture of the acetins. The solvent system ethyl acetate/n-butanol/water 1:0.2:1 was used in all separation procedures. Mono-, di- and triacetins were separated similarly in the semi-preparative and preparative runs.
在这项工作中，我们报告了通过逆流色谱法将粗乙酸混合物纯化为单、二和三乙酸酯。该过程最初在小的半制备规模（0.5g）上进行测试，以确定其效率。然后，该过程扩大到可容纳 2.5g 的粗反应产物，其中含有乙酸酯混合物。在所有分离过程中均使用乙酸乙酯/正丁醇/水 1:0.2:1 的溶剂系统。单、二和三乙酸酯在半制备和制备运行中也类似地分离。
