[具有腐蚀性细菌的胞外聚合物在钢表面生物膜形成中的作用]

[Role of exopolymeric substances of corrosion-aggressive bacteria in the biofilm formation on the steel surface].

作者信息

Purish L M, Asaulenko L H, Abdulina D R, Vasyl'ev V M, Iutyns'ka H O

出版信息

Mikrobiol Z. 2011 Jan-Feb;73(1):3-9.

Abstract

It had been done the comparative study of the exopolymeric substances (EPS) synthesized by the sulfidogenic microbial community and monocultures of Desulfovibrio sp. 10, Bacillus subtilis 36 and Pseudomonas aeruginosa 27 under various growth models as biofilm and plankton was performed. It was established that biofilm-produced exopolymers contained increased amount of glucose and fucose, while planktonic ones had more amount of mannose and rhamnose. The amount of rhamnose was 24% of the total amount of carbohydrates in the planktonic-produced exopolymers synthesized by Pseudomonas aeruginosa 27 and Bacillus subtilis 36. Glucuronic acid, galactosamine and glucosamine along with neutral carbohydrates were found in the composition of EPS synthesized by associative cultures, while only galactosamine was found in EPS synthesized by Desulfovibrio sp. 10. The amount of hexuronic acids and hexozamines was, respectively, 4.6 and 1.6 times higher in the biofilm formed by Pseudomonas aeruginosa 27 on the steel surface, than in the planktonic exopolymers. It is discussed the role in the biofilm formation of dominative members of the corrosion-aggressive microbial community.

摘要

对产硫化物微生物群落以及脱硫弧菌属10、枯草芽孢杆菌36和铜绿假单胞菌27的纯培养物在生物膜和浮游生物等不同生长模式下合成的胞外聚合物（EPS）进行了比较研究。结果表明，生物膜产生的胞外聚合物中葡萄糖和岩藻糖含量增加，而浮游生物产生的胞外聚合物中甘露糖和鼠李糖含量更多。鼠李糖在铜绿假单胞菌27和枯草芽孢杆菌36合成的浮游生物产生的胞外聚合物中的含量占碳水化合物总量的24%。在联合培养物合成的EPS成分中发现了葡萄糖醛酸、半乳糖胺和葡萄糖胺以及中性碳水化合物，而在脱硫弧菌属10合成的EPS中仅发现了半乳糖胺。铜绿假单胞菌27在钢表面形成的生物膜中己糖醛酸和己糖胺的含量分别比浮游胞外聚合物高4.6倍和1.6倍。文中讨论了具有腐蚀活性的微生物群落中优势成员在生物膜形成中的作用。