Purishch L M, Asaulenko L H, Koptieva Zh P, Kozlova I P
Mikrobiol Z. 2004 Jul-Aug;66(4):78-85.
It was shown in the laboratory investigations that the cells of sulphate-reducing bacteria of both aggressive Desulfovibrio sp. strain Kiev-10 and nonaggressive Desulfovibrio desulfuricans strain Kiev-45 strains can produce exopolysaccharides (EPS). Plankton (freely floating) cells of sulphate-reducing bacteria produce greater quantity of EPS than the cells of the biofilm formed on steel. The inducing effect of metal on EPS synthesis by sulphate-reducing bacteria has been established. The content of carbohydrates in EPS is higher in the biofilm of aggressive strain Desulfovibrio sp. Kiev-10 than in plankton bacteria as well as compared with the biofilm of less aggressive strain Desulfovibrio desulfuricans Kiev-45. Specific production of EPS of the aggressive strain in the biofilm formed on the steel is 3 orders higher than in the plankton cells. It has been established that the quantity of cells of sulphate-reducing bacteria adhered to steel decreases in the presence of the corrosion inhibitor N-decylpyridinium chloride (high-molecular compound of nitrogen-including PAR of the cationic type). It is shown that EPS synthesis is blocked in the biofilm formed by sulphate-reducing bacteria on steel under the effect of the corrosion inhibitor, while EPS production in plankton is stimulated.
实验室研究表明,具有侵蚀性的脱硫弧菌属基辅-10菌株和非侵蚀性的脱硫脱硫弧菌基辅-45菌株这两种硫酸盐还原菌的细胞都能产生胞外多糖(EPS)。硫酸盐还原菌的浮游(自由漂浮)细胞产生的EPS量比在钢铁上形成的生物膜细胞更多。已经确定了金属对硫酸盐还原菌EPS合成的诱导作用。与浮游细菌相比,以及与侵蚀性较弱的脱硫脱硫弧菌基辅-45菌株的生物膜相比,具有侵蚀性的脱硫弧菌属基辅-10菌株生物膜中EPS的碳水化合物含量更高。在钢铁上形成的生物膜中,侵蚀性菌株的EPS比浮游细胞中的特定产量高3个数量级。已经确定,在存在腐蚀抑制剂N-癸基吡啶氯化物(含氮阳离子型高分子化合物)的情况下,附着在钢铁上的硫酸盐还原菌细胞数量会减少。结果表明,在腐蚀抑制剂的作用下,硫酸盐还原菌在钢铁上形成的生物膜中EPS合成受到阻碍,而浮游状态下的EPS产生则受到刺激。