Edelfors S, Ravn-Jonsen A
Department of Pharmacology, University of Copenhagen, Denmark.
Pharmacol Toxicol. 1990 Jul;67(1):56-60. doi: 10.1111/j.1600-0773.1990.tb00782.x.
The effect of various alkanols on the central nervous system was studied by using rat brain synaptosomal membranes as an in vitro model. The activity of (Ca2+/Mg2+)ATPase and the membrane fluidity were determined. The n-alkanols exhibited an increased molar inhibition of the ATPase activity with an increase in the carbon chain length up to 1-octanol. 1-octanol and 1-decanol caused a biphasic effect on the ATPase activity depending on the alkanol concentration, whereas 1-dodecanol caused a stimulation of the ATPase activity. All alkanols studied caused an increased fluidity of the membrane. Our results indicate that the effect of alkanols on the ATPase activity depends on changes in the border layer between the membrane and the surrounding medium and on a binding of the alkanols to the enzyme molecule. Furthermore, the two-way effect of 1-octanol and 1-decanol and the stimulatory effect of 1-dodecanol indicate that more mechanisms are involved. In addition, the results indicate that changes in the membrane fluidity do not seem to be a prerequisite of the ATPase inhibition.
通过使用大鼠脑突触体膜作为体外模型，研究了各种链烷醇对中枢神经系统的影响。测定了（Ca2+/Mg2+）ATP酶的活性和膜流动性。直链烷醇对ATP酶活性的摩尔抑制作用随着碳链长度增加至1-辛醇而增强。1-辛醇和1-癸醇对ATP酶活性产生双相效应，这取决于链烷醇的浓度，而1-十二烷醇则刺激ATP酶活性。所研究的所有链烷醇均导致膜流动性增加。我们的结果表明，链烷醇对ATP酶活性的影响取决于膜与周围介质之间边界层的变化以及链烷醇与酶分子的结合。此外，1-辛醇和1-癸醇的双向效应以及1-十二烷醇的刺激效应表明涉及更多机制。另外，结果表明膜流动性的变化似乎不是ATP酶抑制的先决条件。
