Shakhmatova E I, Bakos P
Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad.
Physiol Bohemoslov. 1990;39(2):157-70.
Detergents (Triton X-100, dodecyl sulphate, saponin) added in concentrations of 0.1-10 mg/ml to solution at the outer frog skin surface reversibly reduced potential difference (PD) and short-circuit current (Isc); when added at the inner surface. Triton X-100 initially induced a short increase in the PD and Isc followed by a pronounced decrease similarly as after the application of any of the detergents used. When added to either serosal or mucosal surface of frog urinary bladder, the detergents reversibly abolished the reactivity to vasopressin. Triton X-100 blocked foskolin and cAMP-induced effects on membrane water permeability. The results suggest that hydrophobic elements of membrane play a crucial role in the regulation of membrane permeability for ions and water and of the responsiveness of the cells to vasopressin. Differences in sensitivity to detergents could be observed between the apical and the basolateral cell membrane.
以0.1 - 10毫克/毫升的浓度添加到青蛙皮肤外表面溶液中的去污剂（曲拉通X - 100、十二烷基硫酸盐、皂角苷）可使电位差（PD）和短路电流（Isc）可逆性降低；当添加到内表面时，曲拉通X - 100最初会使PD和Isc短暂升高，随后显著降低，与使用任何一种去污剂后的情况类似。当添加到青蛙膀胱的浆膜或黏膜表面时，去污剂可使对血管加压素的反应性可逆性消失。曲拉通X - 100可阻断福斯高林和cAMP诱导的对膜水通透性的影响。结果表明，膜的疏水成分在调节离子和水的膜通透性以及细胞对血管加压素的反应性方面起着关键作用。在顶端细胞膜和基底外侧细胞膜之间可观察到对去污剂敏感性的差异。
