Ding Hsiou-Yu, Chang Te-Sheng, Chiang Chien-Min, Shou-Ku Tai Sorgan
Institute of Cosmetics Science, Chia Nan University of Pharmacy and Science, National University of Tainan, Taiwan, ROC.
J Cosmet Sci. 2011 Jan-Feb;62(1):41-8.
The inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells was investigated. The results showed that the P. acidula extract (PAE) inhibited melanogenesis in 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner, with an IC50 value of 33.5 μg/ml. In addition, PAE also inhibited cellular tyrosinase activity. Moreover, western blot and real-time reverse transcriptase polymerase chain reaction (qRT-PCR) analyses respectively confirmed that PAE down-regulated levels of tyrosinase protein and its mRNA in IBMX-stimulated B16 cells. These results demonstrated that PAE inhibits melanogenesis of B16 cells by reducing tyrosinase gene expression. From the present study, PAE is proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation.
研究了酸脚杆水提取物对小鼠B16黑色素瘤细胞黑色素生成的抑制作用。结果表明，酸脚杆提取物（PAE）以剂量依赖的方式抑制3-异丁基-1-甲基黄嘌呤（IBMX）刺激的B16细胞中的黑色素生成，IC50值为33.5μg/ml。此外，PAE还抑制细胞酪氨酸酶活性。此外，蛋白质印迹和实时逆转录聚合酶链反应（qRT-PCR）分析分别证实，PAE下调了IBMX刺激的B16细胞中酪氨酸酶蛋白及其mRNA的水平。这些结果表明，PAE通过降低酪氨酸酶基因表达来抑制B16细胞的黑色素生成。从本研究中可以证明，PAE是一种治疗皮肤色素沉着过度的美白剂的良好候选物。
