Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Neurosci Lett. 2011 May 16;495(2):155-8. doi: 10.1016/j.neulet.2011.03.060. Epub 2011 Apr 2.
An earlier study indicated a possible relationship between Tourette syndrome (TS) and the cytokines. To explore this further, we analyzed the association of the polymorphisms, IL8 -251A/T, IL12B -1188A/C and TNF-α -238A/G, in the IL8, IL12B and TNF-α cytokine genes with TS in a Chinese Han population. A total of 108 patients diagnosed with TS and their parents were recruited for the study. The genetic contributions of the IL8 -251A/T, IL12B -1188A/C, and TNF-α -238A/G polymorphisms were evaluated using polymerase chain reaction and restriction enzyme digestion (PCR-RFLP) and haplotype relative risk (HRR) and transmission disequilibrium test (TDT) statistics. Our results revealed no significant associations between the IL8 -251A/T, IL12B -1188A/C and TNF-α -238A/G polymorphisms and TS (for IL8 -251A/T, TDT=0.418, df=1, P=0.518; HRR=2.17, X(2)=3.000, P=0.083, 95%CI: 0.900-5.230; for IL12B -1188A/C, TDT=1.131, df=1, P=0.288; HRR=1.27, X(2)=0.35, P=0.549, 95%CI: 0.580-2.790; for TNF-α -238A/G, TDT=2.793, df=1, P=0.095; HRR=0.27, X(2)=2.90, P=0.089, 95%CI: 0.061-1.217). This result was confirmed using haplotype-based haplotype relative risk (HHRR) which allows the two alleles in each genotype to be considered separately. Our data suggests that the IL-8 -251A/T, IL-12B -1188A/C and TNF-α -238A/G polymorphisms may not be associated with susceptibility to TS in the Chinese Han population studied. However, these results need to be replicated using larger datasets collected from different populations.
先前的研究表明妥瑞氏症（TS）与细胞因子之间可能存在关联。为了进一步探索这一点，我们分析了白细胞介素 8（IL8）-251A/T、白细胞介素 12B（IL12B）-1188A/C 和肿瘤坏死因子-α（TNF-α）-238A/G 基因中的多态性与中国汉族人群 TS 之间的关联。我们招募了 108 名被诊断为 TS 的患者及其父母进行这项研究。使用聚合酶链反应和限制性内切酶消化（PCR-RFLP）以及单体型相对风险（HRR）和传递不平衡检验（TDT）统计方法评估了 IL8-251A/T、IL12B-1188A/C 和 TNF-α-238A/G 多态性的遗传贡献。我们的结果显示，IL8-251A/T、IL12B-1188A/C 和 TNF-α-238A/G 多态性与 TS 之间无显著关联（对于 IL8-251A/T，TDT=0.418，df=1，P=0.518；HRR=2.17，X(2)=3.000，P=0.083，95%CI：0.900-5.230；对于 IL12B-1188A/C，TDT=1.131，df=1，P=0.288；HRR=1.27，X(2)=0.35，P=0.549，95%CI：0.580-2.790；对于 TNF-α-238A/G，TDT=2.793，df=1，P=0.095；HRR=0.27，X(2)=2.90，P=0.089，95%CI：0.061-1.217）。使用单体型相对风险（HHRR）进行的基于单体型的验证确认了这一结果，HHRR 允许单独考虑每个基因型中的两个等位基因。我们的数据表明，白细胞介素 8（IL-8）-251A/T、白细胞介素 12B（IL-12B）-1188A/C 和肿瘤坏死因子-α（TNF-α）-238A/G 多态性可能与中国汉族人群 TS 的易感性无关。然而，这些结果需要使用来自不同人群的更大数据集进行复制。
