Mikshis N I, Popova P Iu, Kudriavtseva O M, Goncharova A Iu, Popov Iu A, Kutyrev V V
Zh Mikrobiol Epidemiol Immunobiol. 2011 Jan-Feb(1):44-8.
To study the ability of recombinant protective antigen (PA) to stimulate adaptive immune response in laboratory animals.
Vaccine, recombinant, and reference strains of Bacillus anthracis were used in the study. Laboratory animals were immunized subcutaneously with two doses of antigenic preparation or one dose of B. anthracis strain. After inoculation with reference strain of B. anthracis, measurement of LD50 as well as indexes of immunity was performed by specified methods.
It was revealed that asporogenic recombinant strain has stable biological characteristics during passages in vitro and is effective producer of PA. Using 2-stage chromatography, highly purified protein was obtained. Experiments on different biomodels--BALB/c mice, guinea pigs, and rabbits--demonstrated high protective activity of PA obtained from asporogenic producer. Increase of immunity index was noted when EA1 protein from S-layer was added to preparation for immunization.
Immunity indexes determined in experiments on laboratory animals point to high protective efficacy of recombinant PA. Further studies of its interaction with macroorganism's innate and adaptive immunity systems are promising.
研究重组保护性抗原（PA）刺激实验动物适应性免疫反应的能力。
本研究使用了炭疽芽孢杆菌的疫苗株、重组株和参考株。实验动物皮下注射两剂抗原制剂或一剂炭疽芽孢杆菌菌株。接种炭疽芽孢杆菌参考株后，采用特定方法测定半数致死量（LD50）以及免疫指标。
结果表明，无芽孢重组菌株在体外传代过程中具有稳定的生物学特性，是PA的有效产生菌。通过两步层析法获得了高度纯化的蛋白。在不同生物模型——BALB/c小鼠、豚鼠和兔子——上进行的实验表明，从无芽孢产生菌获得的PA具有很高的保护活性。当将S层的EA1蛋白添加到免疫制剂中时，免疫指标有所提高。
在实验动物实验中测定的免疫指标表明重组PA具有很高的保护效力。对其与机体固有和适应性免疫系统相互作用的进一步研究具有广阔前景。
