Wright Rick, Mackey R Brian, Silva Matthew, Steger-May Karen
Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri 63131, USA.
J Knee Surg. 2010 Dec;23(4):193-9. doi: 10.1055/s-0030-1268695.
Cigarette smoking has been shown to delay soft tissue healing and complicate musculoskeletal recovery. Its deleterious effects have been proven in bone and soft tissue, although to a much more limited fashion in ligament or tendon. We exposed 120 experimental mice to two cigarettes per day for 2 months and then induced a blunt medial collateral ligament (MCL) injury. Mice were assigned to three groups: the first group exposed to two cigarettes per day after injury, the second group exposed to one cigarette per day after injury, and the third group not exposed after injury. A fourth, no-smoking group served as control. The cut and contralateral intact ligaments were biomechanically tested to failure at 7 and 28 days. The ligaments of mice exposed to cigarette smoke were weaker (p = 0.02) and less stiff (p = 0.0004) at 28 days (3.2 N, 3.5 N/mm) compared with those of mice exposed to cigarette smoke for 7 days (3.9 N, 4.7 N/mm). The ligaments of mice exposed to cigarette smoke were weaker (p = 0.02) and less stiff (p = 0.01) at 28 days compared with control mice at 28 days. Between 7 and 28 days, smoking had a deleterious effect on healing manifested as weaker and less stiff ligaments. However, our findings did not support a dose-dependent effect of cigarette exposure on the tensile mechanical properties of ligaments.
吸烟已被证明会延迟软组织愈合，并使肌肉骨骼恢复复杂化。其有害影响在骨骼和软组织中已得到证实，尽管在韧带或肌腱中的影响程度要小得多。我们让120只实验小鼠每天吸食两支香烟，持续2个月，然后造成钝性内侧副韧带（MCL）损伤。小鼠被分为三组：第一组在受伤后每天吸食两支香烟，第二组在受伤后每天吸食一支香烟，第三组在受伤后不吸烟。第四组为不吸烟组作为对照。在第7天和第28天对切断的和对侧完整的韧带进行生物力学测试直至失效。与吸食香烟7天的小鼠（3.9 N，4.7 N/mm）相比，吸食香烟28天的小鼠韧带在第28天更弱（p = 0.02）且刚度更低（p = 0.0004）（3.2 N，3.5 N/mm）。与第28天的对照小鼠相比，吸食香烟28天的小鼠韧带更弱（p = 0.02）且刚度更低（p = 0.01）。在第7天至第28天之间，吸烟对愈合有有害影响，表现为韧带更弱且刚度更低。然而，我们的研究结果不支持香烟暴露对韧带拉伸力学性能的剂量依赖性影响。
