The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Hospital and School of Stomatology, Wuhan University, Wuhan, China.
Angle Orthod. 2011 Jul;81(4):600-8. doi: 10.2319/102510-622.1. Epub 2011 Mar 25.
To test the hypothesis that leukemia inhibitor factor (LIF) and LIF receptor (LIFR) are expressed in periodontal tissue and that their expression may be upregulated during orthodontic tooth movement.
Forces of 0.3 N were applied to move the upper left first molars mesially in 24 rats. These forces were kept constant for 3, 7, and 14 days and followed by animal sacrifice. The contralateral molars served as control. The rate of tooth movement was measured by Image J software. Paraffin-embedded sections of the upper jaws were prepared for histological and immunohistochemical analysis to test the LIF and LIFR expression.
Loaded teeth showed a significantly higher rate of tooth movement. The periodontium of the moved teeth experienced tissue remodeling, while there was no obvious change in the contralateral controls. Furthermore, LIF and LIFR were expressed in the periodontal tissue, and there were statistically significant differences between the loaded and unloaded teeth at 3 and 14 days. LIF presented significantly higher expression on the tension side compared with the pressure side at 3 days.
Both LIF and LIFR exist in the periodontal tissue, and continuous orthodontic forces induce the upregulation of LIF/LIFR production, suggesting that LIF/LIFR may play important roles in periodontium remodeling.
验证成纤维细胞生长因子 9（LIF）及其受体（LIFR）在牙周组织中表达，以及在正畸牙移动过程中其表达可能上调的假说。
将 24 只大鼠左侧上颌第一磨牙向近中施以 0.3N 的力。这些力持续 3、7 和 14 天，然后处死动物。对侧磨牙作为对照。通过 Image J 软件测量牙齿移动速度。制备上颚石蜡包埋切片进行组织学和免疫组织化学分析，以检测 LIF 和 LIFR 的表达。
负载牙齿的牙齿移动速度明显更高。移动牙齿的牙周组织经历了组织重塑，而对侧对照牙齿没有明显变化。此外，LIF 和 LIFR 存在于牙周组织中，在加载和未加载牙齿之间在 3 天和 14 天时有统计学差异。在 3 天时，LIF 在张应力侧的表达明显高于压应力侧。
LIF 和 LIFR 均存在于牙周组织中，持续的正畸力诱导 LIF/LIFR 的产生上调，提示 LIF/LIFR 可能在牙周组织重塑中发挥重要作用。
