2-Color encoding solid phase minisequencing.

Solid phase minisequencing is an effective single nucleotide determination technique. However, two main methods currently used are both time and money consuming. Here, we introduced a more economical and time efficient approach, called 2-color encoding minisequencing, in which four varieties of nucleotides were labeled by the combination of two fluorescence dyes. Such code was sensitive for different nucleotides, and the sequencing results were highly uniform and repeatable.