Dauchel H, Julen N, Lemercier C, Daveau M, Ozanne D, Fontaine M, Ripoche J
INSERM U-78, Bois-Guillaume, France.
Eur J Immunol. 1990 Aug;20(8):1669-75. doi: 10.1002/eji.1830200808.
We have studied the secretion of proteins of the alternative pathway of complement C3, factor B and factor H by human umbilical vein endothelial cells (HUVEC). Results showed that factor H and factor B are quantitatively secreted in abundance whereas C3 could only be detected when the cells are maintained in culture during long periods of time. Interferon-gamma stimulated factor H, factor B and, to a lesser extent, C3 secretions. Interleukin (IL) 1 had a differential effect on spontaneous C3, factor B and factor H secretions. In the presence of IL 1, there was a significant secretion of C3 occurring within a short period of culture. IL 1 also stimulated factor B secretion. There was a synergistic stimulating effect between IL 1 and interferon-gamma to bring C3 and factor B productions by HUVEC to very high levels. In contrast, factor H secretion was consistently inhibited by IL 1. Local increase in C3 and factor B secretions by endothelial cells in the presence of IL 1 may have important implications in the inflammatory reaction. In striking contrast, the glucocorticoid dexamethasone (DXM) had modulatory effects which are consistent with its anti-inflammatory properties. DXM, at therapeutic concentrations, decreased C3 and factor B secretions and increased factor H secretion. Local modulation of complement protein secretion by DXM appears to be a new mechanism by which this glucocorticoid may control inflammation.
我们研究了人脐静脉内皮细胞（HUVEC）对补体C3替代途径中的蛋白、B因子和H因子的分泌情况。结果显示，H因子和B因子大量定量分泌，而只有当细胞长期维持在培养状态时才能检测到C3。γ干扰素刺激了H因子、B因子的分泌，对C3的分泌也有一定程度的刺激。白细胞介素（IL）-1对自发的C3、B因子和H因子分泌有不同的影响。在存在IL-1的情况下，培养短期内C3有显著分泌。IL-1也刺激了B因子的分泌。IL-1和γ干扰素之间存在协同刺激作用，使HUVEC产生的C3和B因子达到非常高的水平。相反，IL-1持续抑制H因子的分泌。在存在IL-1的情况下内皮细胞C3和B因子分泌的局部增加可能在炎症反应中具有重要意义。与之形成鲜明对比的是，糖皮质激素地塞米松（DXM）具有与其抗炎特性相符的调节作用。治疗浓度的DXM减少了C3和B因子的分泌，增加了H因子的分泌。DXM对补体蛋白分泌的局部调节似乎是这种糖皮质激素控制炎症的一种新机制。
