Department of Neurobiology, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
J Comp Neurol. 2011 Jul 1;519(10):1970-8. doi: 10.1002/cne.22616.
The present study examined the effects of Reelin in the migration of sympathetic preganglionic neurons (SPN) in the spinal cord of the chick. SPN in the chick first migrate from the neuroepithelium to the ventrolateral spinal cord. They then undergo a secondary migration to cluster adjacent to the central canal, forming the column of Terni (CT). During secondary migration, abundant Reelin is found in large areas of the ventral spinal cord; the only areas devoid of Reelin are areas occupied by SPN or somatic motor neurons and the pathway along which SPN migrate. Ectopic expression of Reelin in the pathway of SPN through electroporation of full-length Reelin DNA stopped SPN migration toward their destination. The spatiotemporal pattern of Reelin expression, along with the inhibition of SPN migration by exogenous Reelin, suggests that Reelin functions as a barrier to SPN migration during normal development of the spinal cord.
本研究探讨了 Reelin 在鸡脊髓交感节前神经元（SPN）迁移中的作用。鸡的 SPN 首先从神经上皮迁移到腹外侧脊髓。然后，它们进行二次迁移，聚集在中央管附近，形成特尔尼柱（CT）。在二次迁移过程中，大量的 Reelin 存在于腹侧脊髓的大片区域中；唯一没有 Reelin 的区域是 SPN 或躯体运动神经元占据的区域以及 SPN 迁移的途径。通过全长 Reelin DNA 的电穿孔在外源性 SPN 途径中异位表达 Reelin 阻止了 SPN 向其目的地迁移。Reelin 表达的时空模式，以及外源性 Reelin 对 SPN 迁移的抑制作用，表明 Reelin 在脊髓的正常发育过程中作为 SPN 迁移的屏障发挥作用。
