Institute of Chemistry, UNICAMP, PO Box 6154, 13083-970 Campinas, SP, Brazil.
Magn Reson Chem. 2011 Jun;49(6):295-300. doi: 10.1002/mrc.2740. Epub 2011 Mar 31.
A better comprehension of the prilocaine (PLC)-β-cyclodextrin (β-CD) complex liberation to membranes was provided by studying the architectural supramolecular arrangements of PLC, β-CD and egg phosphatidylcholine (EPC) liposomes, a membrane model. The topologies and possible interactions of mixtures of PLC, β-CD and EPC liposomes were investigated by nuclear magnetic resonances combining experimental (1)H-NMR (1D ROESY, STD and DOSY) at different pHs. The results indicate that in the mixture PLC/β-CD/EPC at pH 10 the PLC molecules are almost totally embedded into the liposomes and little interaction was observed between PLC and β-CD. However, at pH 5.5 not only was PLC imbedded in the EPC bilayer, but PLC was also interacting with β-CD. These results were rationalized as a spontaneous PLC release from β-CD to liposomes vesicles, whereas the PLC/EPC complex formation was higher at pH 10 than pH 5.5.
通过研究普鲁卡因（PLC）-β-环糊精（β-CD）复合物向膜的释放，更好地理解了其超分子结构排列。以卵磷酯（EPC）脂质体作为膜模型，研究了 PLC、β-CD 和 EPC 脂质体混合物的拓扑结构和可能的相互作用。通过核磁共振结合实验（1）H-NMR（1D ROESY、STD 和 DOSY）在不同 pH 值下研究了混合物 PLC/β-CD/EPC 的情况。结果表明，在 pH 值为 10 的混合物 PLC/β-CD/EPC 中，PLC 分子几乎完全嵌入脂质体中，并且在 PLC 和β-CD 之间观察到很少的相互作用。然而，在 pH 值为 5.5 时，不仅 PLC 嵌入 EPC 双层，而且 PLC 还与β-CD 相互作用。这些结果可以解释为 PLC 从β-CD 自发释放到脂质体囊泡中，而在 pH 值为 10 时，PLC/EPC 复合物的形成高于 pH 值为 5.5 时。
