Departament de Producció Animal, Universitat de Lleida, Rovira Roure 191, 25198, Lleida, Spain.
Rapid Commun Mass Spectrom. 2011 Apr 30;25(8):1082-8. doi: 10.1002/rcm.4958. Epub 2011 Mar 29.
In some Mediterranean products such as olive oil or ham, oleic acid is the most abundant component of the total fat. Due to the large volume of trade in these products, it may be necessary to analyze oleic fatty acids in high numbers of samples in short periods of time. However, using classic lipid analysis techniques, it is not always possible to cope with these high demands. To solve this problem, a high-throughput analytical method for oleic fatty acid quantification in pork is presented. The purpose of the method is to avoid liquid chromatography processes using a flow injection analysis (FIA) system based on electrospray ionization mass spectrometry. The use of pentadecanoic fatty acid as an internal standard overcame matrix effects. The oleic FIA technique could be used as a suitable method for discriminating carcass samples for selection and labeling by oleic acid content when large numbers of pork samples must be processed in a short period of time.
在一些地中海产品中，如橄榄油或火腿，油酸是总脂肪中最丰富的成分。由于这些产品的交易量很大，可能需要在短时间内分析大量样品中的油酸脂肪酸。然而，使用经典的脂质分析技术，并不总是能够满足这些高要求。为了解决这个问题，提出了一种高通量分析方法来定量猪肉中的油酸脂肪酸。该方法的目的是避免使用基于电喷雾电离质谱的流动注射分析（FIA）系统进行液相色谱过程。使用十五烷酸作为内标克服了基质效应。油酸 FIA 技术可作为一种合适的方法，用于在短时间内处理大量猪肉样品时，根据油酸含量对胴体样品进行选择和标记。
