Hu Jianguo, Zhou Jiansheng, Li Xingwu, Wang Fengchao, Lü Hezuo
Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road Bengbu 233004, PR China.
Indian J Exp Biol. 2011 Mar;49(3):177-82.
The transplantation of Schwann cells (SCs) could successfully promote axonal regeneration. This is likely to attribute to the adhesion molecules expression and growth factors secretion of SCs. But which factor(s) play a key role has not been precisely studied. In this study, an outgrowth assay using dorsal root ganglia (DRG) neuron-SC co-culture system in vitro was performed. Co-culture of SCs or application of SC-conditioned medium (CM) substantially and significantly increased DRG neurite outgrowth. Further, nerve growth factor and NGF receptor (TrkA) mRNA were highly expressed in Schwann cells and DRG neuron, respectively. The high concentration of NGF protein was detected in SC-CM. When K-252a, a specific inhibitor of NGF receptor was added, DRG neurite outgrowth was significantly decreased in a concentration-dependent manner. These data strongly suggest that SCs play important roles in neurite outgrowth of DRG neurons by secreted NGF.
雪旺细胞（SCs）的移植能够成功促进轴突再生。这可能归因于雪旺细胞的黏附分子表达和生长因子分泌。但究竟是哪种因素起关键作用尚未得到精确研究。在本研究中，进行了一项利用背根神经节（DRG）神经元 - 雪旺细胞共培养系统的体外生长试验。雪旺细胞的共培养或雪旺细胞条件培养基（CM）的应用显著且明显地增加了背根神经节神经突的生长。此外，神经生长因子和NGF受体（TrkA）mRNA分别在雪旺细胞和背根神经节神经元中高表达。在雪旺细胞条件培养基中检测到高浓度的NGF蛋白。当加入NGF受体的特异性抑制剂K - 252a时，背根神经节神经突的生长以浓度依赖的方式显著降低。这些数据有力地表明，雪旺细胞通过分泌NGF在背根神经节神经元的神经突生长中发挥重要作用。
