Research Unit of Clinical Microbiology, Institute of Clinical Research, University of Southern Denmark, Odense C, Denmark.
Biomaterials. 2011 Jul;32(20):4481-8. doi: 10.1016/j.biomaterials.2011.03.002. Epub 2011 Mar 31.
In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90% reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.
在当前的研究中，我们研究了血液补体在医疗器械硅橡胶上的激活情况，并提出了一种等离子体聚合的乙烯基吡咯烷酮（ppVP）涂层，该涂层强烈降低了血液补体系统的表面激活。我们表明，未涂层的硅橡胶和聚苯乙烯都是补体系统的有效激活剂，这可以通过激活、沉积的 C3b 和定量测定的裂解片段 C3c 的流体相释放来衡量。与未处理的硅橡胶相比，ppVP 涂层的硅橡胶的补体激活减少了约 90%。石英晶体微天平（QCM-D）测量表明，血液蛋白（包括天然 C3）相对强烈地吸附到 ppVP 表面，这表明补体激活的减少既不是由于蛋白质吸附低，也不是由于直接的 C3 结合减少，因此可能是吸附蛋白层组成差异的结果。替代和经典的补体途径在 ppVP 上几乎检测不到，而通过 MBL/ficolin-2 沉积的凝集素途径在 ppVP 上仍然保持活性，这表明该途径是 ppVP 涂层表面剩余的微妙激活的原因。ppVP 表面还使用扫描电子显微镜（SEM）、X 射线光电子能谱（XPS）和傅里叶变换红外（FTIR）进行了物理和化学表征，这表明所应用的等离子体处理保留了化学功能。总的来说，ppVP 涂层具有增加血液接触设备的补体相容性的潜力。
