Ali Niaz, Ali Shah Syed Wadood
Department of Pharmacy, University of Malakand, Pakistan.
Pak J Pharm Sci. 2011 Apr;24(2):171-4.
The aim of the study was to explore the traditional antispasmodic use of Teucrium stocksianum on scientific grounds, and preliminary screen the specie for phytochemical constituents. Crude methanolic extract of aerial parts of Teucrium stocksianum (Ts.Cr) was studied for possible relaxant effects on spontaneous rabbits' jejunum preparations at concentrations of 0.01, 0.03, 0.1, 0.3, 3.0, 5.0 and 10.0 mg/ml. Effects of Ts.Cr was also studied on 80 mM KCl-induced contractions to find out possible mechanism as antispasmodic. Calcium chloride curves were constructed in the presence and absence of Ts.Cr at different concentrations in decalcified rabbits' jejunum preparations and were compared with curves of verapamil. Plant was also screened for different classes of phytochemicals. The plant gave positive tests for the presence of different classes of phytochemicals like carbohydrates, proteins and amino acids, saponins, tannins, flavonoids and sterols. It gave negative tests for alkaloids, anthraquinone glycosides and cardiac glycosides. Ts.Cr caused 100% relaxation of the spontaneous rabbit's jejunum preparations at dose of 5.0 mg/ml (EC50=1.98 mg/ml±0.07, n=6). Contractions induced by 80 mM potassium chloride (KCl) were also relaxed 100% by Ts.Cr at dose of 5.0 mg/ml. When tested for possible calcium channel blockade in calcium free K+-rich medium, Ts.Cr at concentration of 0.3 mg/ml produced a right shift in the calcium chloride curves vs. control (log EC50=-1.78±0.03 [Ca++] M vs. control log EC50=-2.57±0.07 [Ca++] M). In similar fashion, a right shift was observed for the calcium chloride curves in the tissues treated with 0.1 μM verapamil. When tested on histamine and barium chloride induced contractions, the extract produced no significant effect. The results confirm the folkloric use of Teucrium stocksianum as antispasmodic possibly through the calcium channel blocking mechanism.
本研究的目的是从科学角度探索斯托克斯香科科（Teucrium stocksianum）的传统解痉用途，并对该物种的植物化学成分进行初步筛选。研究了斯托克斯香科科地上部分的粗甲醇提取物（Ts.Cr）在浓度为0.01、0.03、0.1、0.3、3.0、5.0和10.0mg/ml时对家兔空肠自发收缩的可能松弛作用。还研究了Ts.Cr对80mM氯化钾诱导的收缩的影响，以找出其作为解痉剂的可能作用机制。在脱钙家兔空肠标本中，在有无不同浓度Ts.Cr的情况下构建氯化钙曲线，并与维拉帕米曲线进行比较。还对该植物进行了不同类别的植物化学成分筛选。该植物对不同类别的植物化学成分如碳水化合物、蛋白质和氨基酸、皂苷、单宁、黄酮类化合物和甾醇呈阳性反应。对生物碱、蒽醌苷和强心苷呈阴性反应。Ts.Cr在5.0mg/ml剂量时可使家兔空肠自发收缩制剂100%松弛（EC50=1.98mg/ml±0.07，n=6）。5.0mg/ml剂量的Ts.Cr也可使80mM氯化钾诱导的收缩100%松弛。在无钙高钾培养基中测试其可能的钙通道阻滞作用时，0.3mg/ml浓度的Ts.Cr使氯化钙曲线相对于对照组右移（log EC50=-1.78±0.03[Ca++]M，对照组log EC50=-2.57±0.07[Ca++]M）。以类似方式，在用0.1μM维拉帕米处理的组织中，氯化钙曲线也出现右移。在组胺和氯化钡诱导的收缩实验中，该提取物无显著作用。结果证实了斯托克斯香科科作为解痉剂的民间用途可能是通过钙通道阻滞机制实现的。
