Department of Physiology, Georgia Health Science University, GA, USA.
Biol Res Nurs. 2011 Jul;13(3):227-34. doi: 10.1177/1099800411402494. Epub 2011 Mar 30.
Scientists and clinicians frequently use immunological methods (IMs) to investigate complex biological phenomena. Commonly used IMs include immunocytochemistry (IC), enzyme-linked immunosorbent assays (ELISA) and flow cytometry. Each of these methodologies exploits a common principle in IMs -the binding of an antibody to its antigen. Scientists continue to develop new methodologies, such as high-throughput immunohistochemistry (IHC) and in vivo imaging techniques, which exploit antibody-antigen binding, to more accurately answer complex research questions involving single cells up to whole organ systems. The purpose of this paper is to discuss established and evolving IMs and to illustrate the application of these methods to nursing research.
科学家和临床医生经常使用免疫学方法 (IMs) 来研究复杂的生物现象。常用的 IMs 包括免疫细胞化学 (IC)、酶联免疫吸附测定 (ELISA) 和流式细胞术。这些方法学中的每一种都利用了 IMs 中的一个共同原理——抗体与其抗原的结合。科学家们继续开发新的方法学，例如高通量免疫组织化学 (IHC) 和体内成像技术，利用抗体-抗原结合，更准确地回答涉及单细胞到整个器官系统的复杂研究问题。本文的目的是讨论已建立和不断发展的 IMs，并说明这些方法在护理研究中的应用。
