Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, USA.
Plant Signal Behav. 2011 May;6(5):719-22. doi: 10.4161/psb.6.5.15104. Epub 2011 May 1.
In animals, Tandem CCCH Zinc Finger (TZF) proteins can affect gene expression at both transcriptional and post-transcriptional levels. In Arabidopsis thaliana, AtTZF1 is a member of the TZF family characterized by a plant-unique tandem zinc finger motif. AtTZF1 can bind both DNA and RNA in vitro, and it can traffic between the nucleus and cytoplasmic foci. However, no in vivo DNA/RNA targets have been identified so far, and little is known about the molecular mechanisms underlying AtTZF1's profound effects on plant growth, development, and stress responses. In order to determine whether AtTZF1 can function as a transcription factor, transactivation assays were conducted. Results indicated that AtTZF1 fusion proteins could not exert obvious transcriptional activity in a maize protoplast transient expression system. However, this conclusion might be biased due to poor nuclear localization of AtTZF1 fusion proteins in the assay system.
在动物中，串联 CCCH 锌指（TZF）蛋白可以在转录和转录后水平上影响基因表达。在拟南芥中，AtTZF1 是 TZF 家族的一员，其特征是具有植物特有的串联锌指基序。AtTZF1 可以在体外结合 DNA 和 RNA，并且可以在核和细胞质焦点之间运输。然而，到目前为止，还没有鉴定出体内 DNA/RNA 的靶标，并且对 AtTZF1 对植物生长、发育和应激反应的深远影响的分子机制知之甚少。为了确定 AtTZF1 是否可以作为转录因子发挥作用，进行了反式激活测定。结果表明，AtTZF1 融合蛋白在玉米原生质体瞬时表达系统中不能发挥明显的转录活性。然而，由于在测定系统中 AtTZF1 融合蛋白的核定位不佳，这一结论可能存在偏差。
