INEB-Instituto de Engenharia Biomédica, Divisão de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
Small. 2011 May 9;7(9):1236-41. doi: 10.1002/smll.201002074. Epub 2011 Mar 29.
The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine.
基于纳米粒子的基因传递系统中靶向分子的密度在其载体性能中起着至关重要的作用。在这里，分子识别力谱被提出作为一种新的筛选工具，以优化功能化纳米粒子的靶向分子密度，从而实现细胞特异性相互作用。通过定制纳米粒子配方，与原子力显微镜尖端相连的纳米粒子之间的解附事件概率与纳米粒子的基因载体能力直接相关。此外，还揭示了蛋白质-受体相互作用的新见解。这种新方法为纳米医学领域开辟了新的途径。
