Lakatosova S, Celec P, Schmidtova E, Kubranska A, Durdiakova J, Ostatnikova D
Comenius University in Bratislava, Faculty of Medicine, Institute of Physiology, Slovakia.
Bratisl Lek Listy. 2011;112(2):58-62.
Reelin plays an important role in the regulation of synaptic plasticity in adulthood. Administration of 5-metoxytryptamine (5MT), an agonist of serotonin receptors, during natal and neonatal periods results in decreased reelin expression. In adulthood, reelin is expressed by GABAergic neurons.
The purpose of this study was to reveal the effect of elevated serotonergic stimulation on the expression of reelin and glutamate decarboxylase (GAD1) in adulthood as well as on depressive behavior and spatial cognitive abilities in adult female rats.
Rats were injected with 5MT. A forced swimming test was used for evaluation of the depressive behavior and Morris water maze test was used for evaluation of spatial cognition. Brains were used for measuring the expression of reelin and GAD1.
We found a significant decrease in reelin expression in the cerebellum and prefrontal cortex of 5MT-treated rats. GAD1 expression was decreased in the cerebellum of 5MT-treated rats. 5MT-treated rats reached a lower immobility score in the forced swimming test. The Morris water maze test did not reveal any significant differences.
We have shown that administration of serotonin receptor agonist resulted in a decreased RELN and GAD1 expression in the cerebellum of adult female rats. We propose that this phenomenon might be relevant in the pathogenesis of autism (Fig. 3, Ref. 38). Full Text in free PDF www.bmj.sk.
Reelin在成年期突触可塑性的调节中起重要作用。在出生期和新生儿期给予5-甲氧基色胺（5MT），一种血清素受体激动剂，会导致Reelin表达降低。在成年期，Reelin由γ-氨基丁酸能神经元表达。
本研究的目的是揭示血清素能刺激升高对成年雌性大鼠成年期Reelin和谷氨酸脱羧酶（GAD1）表达以及抑郁行为和空间认知能力的影响。
给大鼠注射5MT。使用强迫游泳试验评估抑郁行为，使用莫里斯水迷宫试验评估空间认知。取脑测量Reelin和GAD1的表达。
我们发现5MT处理的大鼠小脑和前额叶皮质中Reelin表达显著降低。5MT处理的大鼠小脑中GAD1表达降低。5MT处理的大鼠在强迫游泳试验中静止不动得分较低。莫里斯水迷宫试验未发现任何显著差异。
我们已经表明，给予血清素受体激动剂会导致成年雌性大鼠小脑中RELN和GAD1表达降低。我们认为这种现象可能与自闭症的发病机制有关（图3，参考文献38）。免费PDF全文www.bmj.sk。
