Capon S M, Sacher R A, Deeg H J
Division of Hematology, Georgetown University, Washington, DC.
Transfusion. 1990 Oct;30(8):678-81. doi: 10.1046/j.1537-2995.1990.30891020323.x.
Several plastic materials used in blood storage were evaluated for their ability to transmit ultraviolet B (UVB) light. A plastic bag manufactured from sheets of transparent Teflon efficiently (78-86%) transmitted UVB light and was employed in subsequent functional studies of lymphocytes and platelets exposed to UVB light while contained in these bags. In vitro experiments showed a UVB dose-dependent abrogation of lymphocyte responder and stimulator functions, with concurrent preservation of platelet aggregation responses. In a phase I pilot study, UVB-treated platelet concentrates were administered to four bone marrow transplant recipients. Adverse effects attributable to the transfusions were not observed, and patients showed clinically effective transfusion responses. No patient developed lymphocytotoxic HLA or platelet antibodies. These studies suggest that platelets can be effectively irradiated with UVB light in a closed system. However, numerous variables, including container material, volume and composition of contents, steady exposure versus agitation, and exact UV wavelength, must be considered.
对几种用于血液储存的塑料材料进行了评估，以确定它们传输紫外线B（UVB）光的能力。一种由透明聚四氟乙烯片材制成的塑料袋能高效（78 - 86%）传输UVB光，并被用于后续对装在这些袋子里的淋巴细胞和血小板暴露于UVB光下的功能研究。体外实验表明，UVB对淋巴细胞反应者和刺激者功能的消除呈剂量依赖性，同时血小板聚集反应得以保留。在一项I期初步研究中，将经UVB处理的血小板浓缩物给予了4名骨髓移植受者。未观察到与输血相关的不良反应，且患者显示出临床有效的输血反应。没有患者产生淋巴细胞毒性HLA或血小板抗体。这些研究表明，血小板可以在封闭系统中用UVB光有效照射。然而，必须考虑众多变量，包括容器材料、内容物的体积和成分、持续暴露与搅动以及确切的UV波长。
