das Neves Lais Mara Siqueira, Marcolino Alexandre Marcio, Prado Rodrigo Paschoal, Ribeiro Tiago De Souza, Pinfildi Carlos Eduardo, Thomazini José Antônio
Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, University of São Paulo-FMRP/USP, Ribeirão Preto, Brazil.
Photomed Laser Surg. 2011 Aug;29(8):581-7. doi: 10.1089/pho.2010.2883. Epub 2011 Apr 1.
The purpose of this study was to evaluate the effect of 830-nm laser in blocking the action of nicotine on the viability of skin flap.
The authors have analyzed the deleterious effect of cigarette smoke or nicotine on the skin flap alone with evidence of increased skin necrosis in the flap.
Twenty-four Wistar-albino rats were divided into three groups of eight animals each: Group 1 (control), subjected to a surgical technique to obtain a flap for cranial base, laser irradiation simulation, and a subcutaneous injection of saline; Group 2, similar to Group 1, with subcutaneous injection of nicotine (2 mg/kg/day) for a period of 1 week before and 1 week after surgery; and Group 3, similar to Group 2, with skin flaps subjected to a λ 830-nm laser irradiation. The laser parameters used were: power 30 mW, beam area 0.07 cm(2), irradiance 429 mW/cm(2), irradiation time 84 sec, total energy 2.52 J, and energy density 36 J/cm(2). The laser was used immediately after surgery and for 4 consecutive days, in one point at 2.5 cm of the flap cranial base. The areas of necrosis were examined by two macroscopic analyses: paper template and Mini-Mop(®). The pervious blood vessels were also counted.
The results were statistically analyzed by ANOVA and post-test contrast orthogonal method (multiple comparisons), showing that the laser decreased the area of necrosis in flaps subjected to nicotine, and consequently, increased the number of blood vessels (p < 0.05).
The laser proved to be an effective way to decrease the area of necrosis in rats subjected to nicotine, making them similar to the control group.
本研究旨在评估830纳米激光阻断尼古丁对皮瓣活力影响的效果。
作者已分析香烟烟雾或尼古丁单独对皮瓣的有害作用，有证据表明皮瓣皮肤坏死增加。
24只Wistar白化大鼠被分为三组，每组8只动物：第1组（对照组），采用手术技术获取颅底皮瓣，进行激光照射模拟，并皮下注射生理盐水；第2组，与第1组相似，在手术前1周和手术后1周皮下注射尼古丁（2毫克/千克/天）；第3组，与第2组相似，皮瓣接受波长830纳米的激光照射。所用激光参数为：功率30毫瓦，光斑面积0.07平方厘米，辐照度429毫瓦/平方厘米，照射时间84秒，总能量2.52焦耳，能量密度36焦耳/平方厘米。激光在手术后立即使用，连续使用4天，在距皮瓣颅底2.5厘米处的一个点进行照射。通过两种宏观分析方法检查坏死面积：纸模板法和Mini-Mop®法。同时对通畅血管进行计数。
采用方差分析和事后检验对比正交法（多重比较）对结果进行统计学分析，结果表明激光减少了接受尼古丁处理的皮瓣的坏死面积，从而增加了血管数量（p<0.05）。
事实证明，激光是减少尼古丁处理大鼠坏死面积的有效方法，使其与对照组相似。
