Grendel Tomáš, Sokolský Ján, Vaščáková Andrea, Hrehová Blanka, Poláková Martina, Bobrov Nikita, Sabol František, Gál Peter
Department of Medical Biophysics, Pavol Jozef Šafárik University, Košice, Slovak Republic.
Photomed Laser Surg. 2011 Sep;29(9):613-8. doi: 10.1089/pho.2010.2950. Epub 2011 Apr 1.
The aim of the present study was to evaluate whether LLLT at 830 nm is able to positively modulate trachea incisional wound healing in Sprague-Dawley rats.
Tracheotomy may be associated with numerous complications. Development of excess granulation tissue represents a late complication that may lead to airway occlusion. Low-level laser therapy (LLLT) has been shown to have stimulatory effects on wound healing of different tissues. Therefore, it may be suggested that LLLT could be able to positively modulate trachea wound healing as well.
Using general anesthesia, a median incision was performed from the second to the fifth tracheal cartilage ring in 24 rats. Animals were then randomly divided into sham-irradiated control and laser-treated groups. LLLT (power density: 450 mW/cm(2); total daily dose: 60 J/cm(2); irradiated area ∼1 cm(2)) treatment was performed daily during the first week after surgery. Samples for histological evaluation were removed 7 and 28 days after surgical procedure. Histological sections were stained with hematoxylin-eosin and van Gieson.
Results from our investigation showed that LLLT was able to reduce granulation tissue formation and simultaneously increase new cartilage development at both evaluated time intervals.
From this point of view, LLLT at 830 nm may be a valuable tool in trachea wound healing modulation. Nevertheless, further detailed research is needed to find optimal therapeutic parameters and to test these findings on other animal models.
本研究旨在评估830纳米的低强度激光疗法（LLLT）是否能够对Sprague-Dawley大鼠的气管切开伤口愈合产生积极调节作用。
气管切开术可能会引发多种并发症。过度肉芽组织的形成是一种晚期并发症，可能导致气道阻塞。低强度激光疗法已被证明对不同组织的伤口愈合具有刺激作用。因此，可以推测LLLT也可能对气管伤口愈合产生积极调节作用。
对24只大鼠实施全身麻醉，在第二至第五气管软骨环处做正中切口。然后将动物随机分为假照射对照组和激光治疗组。在手术后的第一周每天进行LLLT治疗（功率密度：450毫瓦/平方厘米；每日总剂量：60焦耳/平方厘米；照射面积约1平方厘米）。在手术7天和28天后取出用于组织学评估的样本。组织学切片用苏木精-伊红和范吉森染色。
我们的研究结果表明，在两个评估时间点，LLLT均能够减少肉芽组织形成，同时增加新软骨的生成。
从这一角度来看，830纳米的LLLT可能是调节气管伤口愈合的一种有价值的工具。然而，需要进一步深入研究以找到最佳治疗参数，并在其他动物模型上验证这些发现。
