Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey.
Theriogenology. 2011 Jul 1;76(1):74-82. doi: 10.1016/j.theriogenology.2011.01.019. Epub 2011 Mar 31.
The aim of the present study was to compare the clinical and endocrinological effects of four different treatments for the induction of abortion in bitches. For this purpose, 28 pregnant bitches between days 25 and 35 of gestation, were randomly assigned to four groups. In group I (n = 7), only aglepristone (AGL, 10mg/kg bw, two injections 24 h apart, s.c.) was administered. In group II (n = 7), AGL (as in group I), cabergolin (CAB, 5 μg/kg, daily p.o., until completion of abortion) and misoprostol (MIS, 200 μg for bitches with ≤ 20 kg bw, 400 μg for bitches with > 20 kg bw, daily intravaginally, until completion of abortion) were administered. In group III (n = 7), AGL (as in group I) and MIS (as in group II) were administered. In group IV (n = 7) AGL, (as in group I) and cloprostenol (CLO, 1μg/kg bw, s.c., two injections 24 h apart with the AGL injections) were combined. In all groups, bitches were examined daily, clinically and ultrasonographically to monitor resorptions/abortions. To measure serum progesterone (P4) and total estrogen (TE) concentrations, blood samples were collected in all groups immediately after the first AGL administration and every other day until completion of abortion. No statistical differences were found between groups concerning the duration until completion of abortion following treatment (n.s.); however, in Group III, 6 d after the start of treatment all pregnancies were terminated whereas in Group I, II and IV, only 57.1% (4/7), 85.7 % (6/7) and 42.8 % (3/7) of pregnancies were terminated. In the latter groups, all pregnancies were terminated between days 8 and 10 after the start of treatment. In Group IV, P4 concentrations on days two and one before the beginning of abortion and the day the abortion started was significantly lower than in the other groups (P < 0.01). No statistical differences were found between groups for TE concentrations (P > 0.05). In Groups I, II and III, no severe side effects occurred. Severe vomiting after each treatment and until the end of abortion was observed in Group IV only. In conclusion, only when a combination of AGL and MIS was used abortion was completed within 6 d in all bitches whereas the additional use of CAB did not improve the treatment.
本研究旨在比较四种不同的犬诱导流产治疗方法的临床和内分泌效果。为此，将 28 只妊娠犬在妊娠第 25 至 35 天随机分配到四组。在第 I 组（n = 7）中，仅给予氨前列醇（AGL，10mg/kg bw，24 小时内两次皮下注射）。在第 II 组（n = 7）中，AGL（与第 I 组相同）、卡麦角林（CAB，5μg/kg，每日口服，直至流产完成）和米索前列醇（MIS，200μg 用于体重≤20kg 的母犬，400μg 用于体重>20kg 的母犬，每日阴道内给药，直至流产完成）。在第 III 组（n = 7）中，给予 AGL（与第 I 组相同）和 MIS（与第 II 组相同）。在第 IV 组（n = 7）中，AGL（与第 I 组相同）和氯前列醇（CLO，1μg/kg bw，皮下注射，与 AGL 注射相隔 24 小时两次注射）联合使用。在所有组中，每天对母犬进行临床和超声检查，以监测吸收/流产情况。为了测量血清孕酮（P4）和总雌激素（TE）浓度，在所有组中，在第一次 AGL 给药后立即采集血液样本，直至流产完成，每隔一天采集一次。治疗后完成流产的时间（n.s.）各组间无统计学差异；然而，在第 III 组，治疗开始后 6 天所有妊娠均终止，而在第 I、II 和 IV 组，仅 57.1%（4/7）、85.7%（6/7）和 42.8%（3/7）的妊娠终止。在后两组中，所有妊娠均在治疗开始后 8 至 10 天终止。在第 IV 组中，流产开始前两天和前一天以及流产开始当天的 P4 浓度明显低于其他组（P<0.01）。各组间 TE 浓度无统计学差异（P>0.05）。在第 I、II 和 III 组中，没有发生严重的副作用。仅在第 IV 组中，在每次治疗后直至流产结束时观察到严重呕吐。结论：只有当 AGL 和 MIS 联合使用时，所有母犬在 6 天内完成流产，而额外使用 CAB 并不能改善治疗效果。
