Department of Oral and Maxillofacial Plastic Surgery, Martin-Luther-University Halle-Wittenberg, Germany.
Int J Oral Maxillofac Surg. 2011 Jul;40(7):737-42. doi: 10.1016/j.ijom.2011.02.020. Epub 2011 Mar 31.
The pathogenesis of cleft lip and palate (CL/P) is studied in animal experiments. This study revealed significant differences in foetal secondary palate development in two strains of mice (NMRI, A/WySnJ) using a palatal organ model. Palatal shelves of 114 NMRI embryos, resistant to cleft occurrence, and 93 A/WySnJ embryos, a strain with a high spontaneous CL/P rate, were micro-dissected at 14.25 GD (gestational day), before palatal fusion takes place. After cultivation in serum-free medium, palatal development was investigated microscopically and scored in a six-step system. At death (14.25 GD) the palatal shelves of the NMRI embryos (mean 3.5) were significant more developed than those of A/WySnJ (mean 2.7; p=0.05). After incubation, 53% (60/114) NMRI and 14% (13/93) A/WySnJ cultures had over two-thirds fusion to stage V-VI, therefore in 17% NMRI (19/114) and 1% A/WySnJ cultures (1/93) fusion was macroscopically complete. 62% of the A/WySnJ cultures showed no significant development in vitro (mean 2.84; p=0.094). There is a significant palatal development difference between normally developed NMRI (mean 4.45, p=0.05) and CL/P appearance in A/WySnJ mice (mean 2.84). Palatal development of both strains was significantly delayed in organ culture (p=0.05). The A/WySnJ strain was more susceptible to manipulation and vulnerable.
腭裂（CL/P）的发病机制在动物实验中进行了研究。本研究使用腭器官模型在两种小鼠品系（NMRI、A/WySnJ）中发现了胎儿腭部发育的显著差异。在腭裂发生抵抗的 114 只 NMRI 胚胎和腭裂自发发生率高的 93 只 A/WySnJ 胚胎的 14.25 GD（妊娠日）时，对腭板进行微解剖，此时腭融合尚未发生。在无血清培养基中培养后，通过显微镜观察和六步评分系统评估腭部发育情况。在死亡（14.25 GD）时，NMRI 胚胎的腭板（平均值 3.5）明显比 A/WySnJ 的更发达（平均值 2.7；p=0.05）。孵育后，53%（60/114）的 NMRI 和 14%（13/93）的 A/WySnJ 培养物融合到 V-VI 期的三分之二以上，因此在 17%的 NMRI（19/114）和 1%的 A/WySnJ 培养物（1/93）中融合是肉眼完全的。62%的 A/WySnJ 培养物在体外没有明显发育（平均值 2.84；p=0.094）。正常发育的 NMRI（平均值 4.45，p=0.05）和 A/WySnJ 小鼠的 CL/P 表现（平均值 2.84）之间存在显著的腭部发育差异。两种品系的腭部发育在器官培养中均显著延迟（p=0.05）。A/WySnJ 品系更易受操作影响，且更脆弱。
