School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Center for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore.
Biosens Bioelectron. 2011 May 15;26(9):3881-6. doi: 10.1016/j.bios.2011.03.002. Epub 2011 Mar 6.
Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10(-13)M, i.e., 100 fM) and selectivity.
还原氧化石墨烯(rGO)-修饰玻碳电极通过电化学阻抗谱法来检测耐甲氧西林金黄色葡萄球菌(MRSA)DNA。我们的实验证实，ssDNA 在与靶 DNA 杂交前后都成功地锚定在 rGO 表面。当预吸附在 rGO 电极上的探针 DNA 与靶 DNA 杂交后，测量的阻抗会显著增加。该方法为高灵敏度（10(-13)M，即 100 fM）和高选择性的 DNA 检测提供了一种新方法。
