The protective function of hydrogen sulfide for lysozyme against riboflavin-sensitized photo-oxidation.

Hydrogen sulfide is the third endogenous signaling gasotransmitter, following nitric oxide and carbon monoxide. Recent studies showed that hydrogen sulfide could alleviate many diseases which were related to the oxidative damage of tissues. It reminded us that hydrogen sulfide might serve as an antioxidant to reduce oxidative pressure. This study showed that hydrogen sulfide protected lysozyme from photo-oxidation induced by riboflavin (RF). Laser flash photolysis was used to explore the mechanisms of antioxidant activity of hydrogen sulfide. The scavenging effects of hydrogen sulfide on the triplet state of riboflavin (³RF(*)) and radicals of tryptophan and tyrosine (TyrO· and TrpN·) were attributed to the protection of lysozyme from photo-oxidation. The results suggested that hydrogen sulfide could serve as an antioxidant in alleviation of oxidative pressure.