Isolation and characterization of a distantly related member of the beta-gamma crystallin super gene family from Xenopus.
作者信息
Shastry B S, Reddy V N
机构信息
Eye Research Institute, Oakland University, Rochester, Michigan 48309.
出版信息
Biochem Biophys Res Commun. 1990 Sep 28;171(3):1333-7. doi: 10.1016/0006-291x(90)90832-8.
PMID:2145835
Abstract
In an attempt to isolate gamma crystallin genes from Xenopus genomic library, we have obtained seventeen clones which strongly hybridized to the gamma 1 cDNA probe from Rana temporaria. Of these four clones are characterized. A restriction map was derived by using single and multiple digests with a variety of enzymes. Hybridization selection of lens mRNAs and in vitro translation produced a protein of 26,000 daltons. These data suggest that the above clones may contain at least part of the gamma or beta crystallin sequences.
摘要
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