口腔炎性肌纤维母细胞瘤的免疫组织化学特征
The immunohistochemical profile of oral inflammatory myofibroblastic tumors.
作者信息
Salgueiredo-Giudice Fernanda, Fornias-Sperandio Felipe, Martins-Pereira Erika, da Costa dal Vechio Aluana Maria, de Sousa Suzana Cantanhede Orsini Machado, dos Santos-Pinto-Junior Décio
机构信息
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Jun;111(6):749-56. doi: 10.1016/j.tripleo.2010.12.023. Epub 2011 Apr 3.
OBJECTIVE
The aim of this study was to demonstrate the immunohistochemical profile of oral inflammatory myofibroblastic tumors (IMTs) along with morphologic analysis.
STUDY DESIGN
Three cases diagnosed as oral IMTs were selected to compile an immunohistochemical panel constituted by calponin, caldesmon, Bcl-2, desmin, fibronectin, CD68, Ki-67, S100, anaplastic lymphoma kinase (ALK), α-smooth muscle actin, cytokeratins AE1/AE3, muscle-specific actin, CD34, and vimentin. An oral squamous cell carcinoma with a focal area of desmoplastic stroma was used as control for the stained myofibroblastic cells.
RESULTS
All oral IMTs were positive for calponin, revealing a strong and diffuse expression in the spindle-shaped cells. The lesions were also positive for vimentin (3/3), fibronectin (3/3), α-smooth muscle actin (3/3), and muscle-specific actin (1/3) and negative for h-caldesmon, Bcl-2, desmin, CD68, Ki-67, S100, ALK, cytokeratins AE1/AE3, and CD34.
CONCLUSIONS
Within the results encountered, the present panel should be of great assistance in the diagnosis of oral IMTs.
目的
本研究旨在通过形态学分析展示口腔炎性肌纤维母细胞瘤（IMTs）的免疫组织化学特征。
研究设计
选取3例诊断为口腔IMTs的病例，构建由钙调蛋白、钙结合蛋白、Bcl-2、结蛋白、纤连蛋白、CD68、Ki-67、S100、间变性淋巴瘤激酶（ALK）、α平滑肌肌动蛋白、细胞角蛋白AE1/AE3、肌特异性肌动蛋白、CD34和波形蛋白组成的免疫组织化学检测组。选取1例具有促纤维增生性基质灶的口腔鳞状细胞癌作为肌纤维母细胞染色的对照。
结果
所有口腔IMTs的钙调蛋白均呈阳性，在梭形细胞中呈强弥漫性表达。病变的波形蛋白（3/3）、纤连蛋白（3/3）、α平滑肌肌动蛋白（3/3）和肌特异性肌动蛋白（1/3）也呈阳性，而h-钙结合蛋白、Bcl-2、结蛋白、CD68、Ki-67、S100、ALK、细胞角蛋白AE1/AE3和CD34呈阴性。
结论
基于所获结果，该检测组对口腔IMTs的诊断有很大帮助。
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