Powell Tremaine B, Tran Phat L, Kim Keesung, Yoon Jeong-Yeol
Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA.
Mater Sci Eng C Mater Biol Appl. 2009 Oct 15;29(8):2459-2463. doi: 10.1016/j.msec.2009.07.010.
A protein nanoarray is created when submicro and nano beads, varying in their size and each conjugated with different proteins, self-assemble to specific locations depending on the diameter matching the surface electron beam patterns created. Protein binding is confirmed from the fluorescence attenuation of the beads upon antigen-antibody binding on the bead surface. This method, called size-dependent self-assembly, allows control of the location of each type of bead, and thus, control of the location of multiple proteins. It provides fast multi-component patterning with a high binding resolution, which can be detected using a fluorescent light microscope. This method is developed to be a simple stand-alone tool for analysis of protein interactions. In addition, it has the potential to be used in conjunction with other methods protein analysis methods, such as enzyme-linked immunosorbent assay (ELISA) and atomic force microscopy (AFM).
当亚微米和纳米珠（其大小各异且各自与不同蛋白质偶联）根据与所产生的表面电子束图案匹配的直径自组装到特定位置时，就形成了蛋白质纳米阵列。通过珠子表面抗原 - 抗体结合后珠子荧光衰减来确认蛋白质结合。这种称为尺寸依赖性自组装的方法能够控制每种类型珠子的位置，进而控制多种蛋白质的位置。它能以高结合分辨率提供快速的多组分图案形成，可使用荧光显微镜进行检测。该方法被开发成为一种用于分析蛋白质相互作用的简单独立工具。此外，它有潜力与其他蛋白质分析方法（如酶联免疫吸附测定法（ELISA）和原子力显微镜（AFM））结合使用。
