培养的人类细胞中端粒平均长度的分析。

Analysis of average telomere length in cultured human cells.

作者信息

Liu Dan

机构信息

Department of Biochemistry and Molecular Biology, Cell-based Assay Screening Service Core, Baylor College of Medicine, Houston, TX, USA.

出版信息

Methods Mol Biol. 2011;735:13-9. doi: 10.1007/978-1-61779-092-8_2.

DOI:10.1007/978-1-61779-092-8_2

PMID:21461807

Abstract

Telomeres play an important role in ensuring the integrity of the genome. Telomere shortening can lead to the loss of genetic information and trigger DNA damage responses. Cultured mammalian cells have served as critical model systems for studying the function of telomere binding proteins and telomerase. Tremendous heterogeneity can be observed both between species and within a single cell line population. Here, we describe the assay that analyzes the average length of telomeres in cultured cells (TRF analysis).

摘要

端粒在确保基因组完整性方面发挥着重要作用。端粒缩短会导致遗传信息丢失并引发DNA损伤反应。培养的哺乳动物细胞已成为研究端粒结合蛋白和端粒酶功能的关键模型系统。在物种之间以及单个细胞系群体内部都能观察到巨大的异质性。在此，我们描述了一种分析培养细胞中端粒平均长度的检测方法（端粒限制片段分析）。