Nantes Cedex, France.
Arch Dermatol Res. 2011 Dec;303(10):707-13. doi: 10.1007/s00403-011-1147-7. Epub 2011 Apr 3.
Acne vulgaris is a skin disease affecting pilosebaceous glands in which Propionibacterium acnes (P. acnes) induced inflammation plays a central role. In order to develop new therapies against the inflammatory events, we evaluated the modulating effect of a new undecyl-rhamnoside, APRC11, on different markers of the inflammation. For this purpose, normal human keratinocytes taken from five healthy donors were pre-incubated for 24 h with APRC11 or Zinc Gluconate (Zn) which was used as reference molecule for its anti-inflammatory properties. Then, keratinocytes were stimulated with P. acnes Membrane Fraction for 6 h, in the presence of either APRC11 or Zn. Different markers were evaluated at mRNA level using a Luminex-based Quantigene array system and at protein level using an ELISA test and a Luminex array system. Results showed that P. acnes significantly increased the expression of IL-1α, IL-1RA, IL-8 and MMP-9. A 24-h treatment with APRC11 prior to the P. acnes stimulation down-regulated the P. acnes-induced cytokines over expression (IL-1α, IL-8 and MMP-9) and up-regulated IL-1RA level in a similar manner than Zn. These regulations were noted at both protein and mRNA levels. In conclusion, the new undecyl-rhamnoside APRC11 is able to down-regulate the expression of molecules implicated in cutaneous inflammation and whose expression is induced by P. acnes, confirming its potential interest in inflammatory acne.
寻常痤疮是一种影响皮脂腺的皮肤疾病，其中痤疮丙酸杆菌（P. acnes）诱导的炎症起着核心作用。为了开发针对炎症事件的新疗法，我们评估了一种新型十一烷基鼠李糖苷 APRC11 对炎症不同标志物的调节作用。为此，从五名健康供体中取出正常的人角质形成细胞，用 APRC11 或锌葡萄糖酸盐（Zn）预孵育 24 小时，Zn 因其抗炎特性而被用作参考分子。然后，在 APRC11 或 Zn 的存在下，用 P. acnes 膜部分刺激角质形成细胞 6 小时。使用基于 Luminex 的 Quantigene 阵列系统在 mRNA 水平和使用 ELISA 测试和 Luminex 阵列系统在蛋白质水平评估不同的标志物。结果表明，P. acnes 显著增加了 IL-1α、IL-1RA、IL-8 和 MMP-9 的表达。在 P. acnes 刺激前用 APRC11 进行 24 小时处理，以类似于 Zn 的方式下调了 P. acnes 诱导的细胞因子过度表达（IL-1α、IL-8 和 MMP-9）并上调了 IL-1RA 水平。这些调节在蛋白质和 mRNA 水平上都得到了证实。总之，新型十一烷基鼠李糖苷 APRC11 能够下调由 P. acnes 诱导的参与皮肤炎症的分子的表达，证实了其在炎症性痤疮中的潜在应用价值。
