INRA, UMR1260 "Nutriments Lipidiques et Prévention des Maladies Métaboliques", Marseille, France.
Mol Nutr Food Res. 2011 Apr;55(4):578-84. doi: 10.1002/mnfr.201000399. Epub 2010 Dec 15.
Carotenoids are mainly stored in adipose tissue. However, nothing is known regarding the uptake of carotenoids by adipocytes. Thus, our study explored the mechanism by which lycopene and lutein, two major human plasma carotenoids, are transported.
CD36 was a putative candidate for this uptake, 3T3-L1 cells were treated with sulfosuccinimidyl oleate, a CD36-specific inhibitor. sulfosuccinimidyl oleate-treated cells showed a significant decrease in both lycopene and lutein uptake as compared to control cells. Their uptake was also decreased by partial inhibition of CD36 expression using siRNA, whereas the overexpression of CD36 in Cos-1 cells increased their uptake. Finally, the effect of CD36 on carotenoid uptake was confirmed ex vivo in cultures of adipose tissue explants from CD36(-/-) mice, which exhibited reduced carotenoid uptake as compared to wild-type mice explants.
For the first time, we report the involvement of a transporter, CD36, in carotenoid uptake by adipocytes and adipose tissue.
类胡萝卜素主要储存在脂肪组织中。然而，目前尚不清楚脂肪细胞对类胡萝卜素的摄取机制。因此，我们的研究探讨了番茄红素和叶黄素这两种主要的人体血浆类胡萝卜素的摄取机制。
CD36 是这种摄取的候选蛋白，用 CD36 特异性抑制剂琥珀酰亚油酰磺酸钠（sulfosuccinimidyl oleate）处理 3T3-L1 细胞。与对照细胞相比，琥珀酰亚油酰磺酸钠处理的细胞中番茄红素和叶黄素的摄取明显减少。使用 siRNA 部分抑制 CD36 的表达也降低了它们的摄取，而在 Cos-1 细胞中过表达 CD36 则增加了它们的摄取。最后，在 CD36（-/-）小鼠的脂肪组织外植体培养物中证实了 CD36 对类胡萝卜素摄取的影响，与野生型小鼠外植体相比，CD36（-/-）小鼠的类胡萝卜素摄取减少。
我们首次报道了一种转运蛋白 CD36 参与了脂肪细胞和脂肪组织对类胡萝卜素的摄取。
