Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan, Republic of Korea.
J Agric Food Chem. 2011 May 25;59(10):5665-70. doi: 10.1021/jf200230s. Epub 2011 Apr 26.
In the present study, an attempt has been made to isolate antiobesity components from crude extracts of the flower buds of Magnolia denudata by CH(2)Cl(2) and MeOH solvents. The crude extracts were partitioned into n-hexane, 85% aqueous MeOH, n-butanol, and water fractions. Their antiobesity effects were evaluated by measuring the effect on adipogenic differentiation using 3T3-L1 cells. Among the fractions, n-hexane and 85% aqueous MeOH fractions effectively reduced the lipid accumulation and the regulation of the adipogenic transcription factor. Both n-hexane and 85% aqueous MeOH fractions were further separated by diverse chromatographic methods to give four lignans (A-D). In comparative analysis, the presence of the lignans during adipogenic differentiation reduced the absorbance values of eluted Oil Red O solution in the order of potency C > D > B > A. Moreover, C and D effectively downregulated SREBP1, PPARγ, and C/EBPα.
在本研究中，尝试用 CH(2)Cl(2)和 MeOH 溶剂从白玉兰花蕾的粗提取物中分离出抗肥胖成分。将粗提取物分成正己烷、85%含水 MeOH、正丁醇和水级分。通过测量对 3T3-L1 细胞的成脂分化的影响来评估它们的抗肥胖作用。在这些级分中，正己烷和 85%含水 MeOH 级分有效地减少了脂肪积累和脂肪生成转录因子的调节。正己烷和 85%含水 MeOH 级分通过多种色谱方法进一步分离，得到四种木脂素（A-D）。在比较分析中，在成脂分化过程中存在木脂素会按效力顺序降低洗脱油红 O 溶液的吸光度值，即 C > D > B > A。此外，C 和 D 有效地下调了 SREBP1、PPARγ 和 C/EBPα。
