Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China.
J Fish Biol. 2011 Apr;78(4):1225-38. doi: 10.1111/j.1095-8649.2011.02929.x.
Three full-length complementary DNA (cDNA) clones were isolated encoding the skeletal myosin light chain 1 (MLC1; 1237 bp), myosin light chain 2 (MLC2; 1206 bp) and myosin light chain 3 (MLC3; 1079 bp) from the fast white muscle cDNA library of mandarin fish Siniperca chuatsi. The sequence analysis indicated that MLC1 and MLC3 were not produced from differentially spliced messenger RNAs (mRNA) as reported in birds and rodents but were encoded by different genes. The MLC2 encodes 170 amino acids, which include four EF-hand (helix-loop-helix) structures. The primary structures of the Ca(2+)-binding domain were well conserved among the MLC2s of seven other fish species. The ontogenetic expression analysis by real-time PCR showed that the three light-chain mRNAs were first detected in the gastrula stage, and their expression increased from the tail bud stage to the larval stage. All three MLC mRNAs showed longitudinal expression variation in the fast white muscle of S. chuatsi, especially MLC1 which was highly expressed at the posterior area. Taken together, the study provides a better understanding about the MLC gene structure and their expression pattern in muscle development of S. chuatsi.
从养殖的大口黑鲈 cDNA 文库中分离得到编码骨骼肌肌球蛋白轻链 1（MLC1；1237bp）、肌球蛋白轻链 2（MLC2；1206bp）和肌球蛋白轻链 3（MLC3；1079bp）的全长 cDNA 克隆。序列分析表明，MLC1 和 MLC3 不是像鸟类和啮齿类动物中报道的那样由差异拼接的信使 RNA（mRNA）产生，而是由不同的基因编码。MLC2 编码 170 个氨基酸，其中包括四个 EF 手（螺旋-环-螺旋）结构。7 种其他鱼类的 MLC2 的 Ca（2+）结合域的一级结构在很大程度上是保守的。实时 PCR 的个体发生表达分析表明，三种轻链 mRNA 首先在原肠胚期检测到，其表达从尾芽期增加到幼虫期。三种 MLC mRNA 在大口黑鲈的快肌中均表现出纵向表达变化，尤其是 MLC1 在后部区域表达水平较高。总之，该研究为了解大口黑鲈肌肉发育中 MLC 基因结构及其表达模式提供了更好的认识。
