Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
J Periodontal Res. 2011 Jun;46(3):354-60. doi: 10.1111/j.1600-0765.2011.01350.x. Epub 2011 Apr 4.
Calprotectin is an important proinflammatory mediator in various inflammatory diseases and is composed of two subunits (S100A8 and S100A9). However, the level of calprotectin in plasma of patients with aggressive periodontitis and its relationship with gene polymorphisms of S100A8 are unclear.
The plasma concentrations of calprotectin were measured, using an enzyme immunoassay, in 139 patients with aggressive periodontitis and in 88 periodontally healthy control subjects. These patients were genotyped for the rs3795391 and rs3806232 polymorphisms of S100A8.
The plasma concentration of calprotectin in patients with aggressive periodontitis was significantly higher than in controls (2.17 mg/L vs. 1.72 mg/L, respectively, p = 0.001). The percentage of the AA genotype of S100A8 rs3795391 was significantly higher in patients than in controls (82% vs. 69.3%, respectively, p = 0.027), while the frequency of the allele G was decreased among patients compared with controls (9.6% vs. 16.1%, respectively, p = 0.036), which was especially apparent in men (rs3795391 genotype, p = 0.005; rs3795391 allele, p = 0.015). The mean probing depth in patients carrying the AA genotype was significantly higher than that of patients carrying the GA + GG genotype of two polymorphisms of S100A8 (rs3795391, p = 0.035; rs3806232, p = 0.040), whereas the levels of calprotectin between different genotypes were not significantly different (rs3795391, p = 0.11; rs3806232, p = 0.15).
These findings indicate that aggressive periodontitis is associated with elevated levels of plasma calprotectin and that gene polymorphisms of S100A8 may influence the susceptibility and severity of aggressive periodontitis.
钙卫蛋白是多种炎症性疾病中的一种重要促炎介质，由两个亚基（S100A8 和 S100A9）组成。然而，侵袭性牙周炎患者血浆中钙卫蛋白的水平及其与 S100A8 基因多态性的关系尚不清楚。
采用酶联免疫吸附法检测 139 例侵袭性牙周炎患者和 88 例牙周健康对照者的血浆钙卫蛋白浓度，并对 S100A8 的 rs3795391 和 rs3806232 多态性进行基因分型。
侵袭性牙周炎患者的血浆钙卫蛋白浓度明显高于对照组（分别为 2.17 mg/L 和 1.72 mg/L，p = 0.001）。S100A8 rs3795391 的 AA 基因型在患者中的比例明显高于对照组（分别为 82%和 69.3%，p = 0.027），而患者中 G 等位基因的频率较对照组降低（分别为 9.6%和 16.1%，p = 0.036），在男性中更为明显（rs3795391 基因型，p = 0.005；rs3795391 等位基因，p = 0.015）。携带两个 S100A8 多态性（rs3795391，p = 0.035；rs3806232，p = 0.040）AA 基因型的患者平均探诊深度明显高于 GA + GG 基因型的患者，而不同基因型之间的钙卫蛋白水平无显著差异（rs3795391，p = 0.11；rs3806232，p = 0.15）。
这些发现表明侵袭性牙周炎与血浆钙卫蛋白水平升高有关，S100A8 基因多态性可能影响侵袭性牙周炎的易感性和严重程度。
