用粒子图像相关光谱（PICCS）定量生物相互作用。
Quantification of biological interactions with particle image cross-correlation spectroscopy (PICCS).
机构信息
Physics of Life Processes, Leiden Institute of Physics, Leiden University, Leiden, The Netherlands.
出版信息
Biophys J. 2011 Apr 6;100(7):1810-8. doi: 10.1016/j.bpj.2010.12.3746.
Abstract
A multitude of biological processes that involve multiple interaction partners are observed by two-color microscopy. Here we describe an analysis method for the robust quantification of correlation between signals in different color channels: particle image cross-correlation spectroscopy (PICCS). The method, which exploits the superior positional accuracy obtained in single-object and single-molecule microscopy, can extract the correlation fraction and length scale. We applied PICCS to correlation measurements in living tissues. The morphogen Decapentaplegic (Dpp) was imaged in wing imaginal disks of fruit fly larvae and we quantified what fraction of early endosomes contained Dpp.
摘要
双色显微镜可观察到涉及多个相互作用伙伴的多种生物过程。在此，我们描述了一种用于稳健定量分析不同颜色通道信号之间相关性的分析方法：粒子图像互相关光谱学（PICCS）。该方法利用单物体和单分子显微镜获得的优越位置精度，可以提取相关分数和长度尺度。我们将 PICCS 应用于活体组织中的相关测量。在果蝇幼虫的翅膀成虫盘（wing imaginal disks）中，我们对形态发生素 Decapentaplegic（Dpp）进行了成像，并定量了早期内体中包含 Dpp 的部分。
相似文献
1
Quantification of biological interactions with particle image cross-correlation spectroscopy (PICCS).用粒子图像相关光谱（PICCS）定量生物相互作用。
Biophys J. 2011 Apr 6;100(7):1810-8. doi: 10.1016/j.bpj.2010.12.3746.
2
Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.Dpp 信号活性需要 Pentagone 与生长中的果蝇翅膀 imaginal 盘组织大小成比例。
PLoS Biol. 2011 Oct;9(10):e1001182. doi: 10.1371/journal.pbio.1001182. Epub 2011 Oct 25.
3
Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc.果蝇翅膀中 Dpp/BMP 形态发生素弥散的非对称需求。
Nat Commun. 2021 Nov 8;12(1):6435. doi: 10.1038/s41467-021-26726-6.
4
Quantification of growth asymmetries in developing epithelia.发育上皮中生长不对称性的量化
Eur Phys J E Soft Matter. 2009 Sep;30(1):93-9. doi: 10.1140/epje/i2009-10507-6. Epub 2009 Sep 18.
5
Dpp spreading is required for medial but not for lateral wing disc growth.Dpp 信号的弥散对于翅芽的中域生长是必需的，但对于侧域生长并非如此。
Nature. 2015 Nov 19;527(7578):317-22. doi: 10.1038/nature15712. Epub 2015 Nov 9.
6
Decapentaplegic signaling regulates Wingless ligand production and target activation during Drosophila wing development.蜕皮激素信号调节果蝇翅膀发育过程中 Wingless 配体的产生和靶标激活。
FEBS Lett. 2020 Apr;594(7):1176-1186. doi: 10.1002/1873-3468.13713. Epub 2019 Dec 25.
7
Formation of the long range Dpp morphogen gradient.长程 Dpp 形态发生素梯度的形成。
PLoS Biol. 2011 Jul;9(7):e1001111. doi: 10.1371/journal.pbio.1001111. Epub 2011 Jul 26.
8
BMP morphogen gradients in flies.果蝇中的 BMP 形态发生梯度。
Cytokine Growth Factor Rev. 2016 Feb;27:119-27. doi: 10.1016/j.cytogfr.2015.11.003. Epub 2015 Nov 24.
9
Patterning of the L2 vein is driven by regulatory interactions between region-specific transcription factors expressed in response to Dpp signalling.L2静脉的模式形成是由响应Dpp信号而表达的区域特异性转录因子之间的调控相互作用驱动的。
Development. 2017 Sep 1;144(17):3168-3176. doi: 10.1242/dev.143461. Epub 2017 Jul 31.
10
Morphogen gradient scaling by recycling of intracellular Dpp.通过细胞内Dpp循环实现形态发生素梯度缩放
Nature. 2022 Feb;602(7896):287-293. doi: 10.1038/s41586-021-04346-w. Epub 2021 Dec 22.
引用本文的文献
1
Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane.利用工程化标签进行四色单分子成像解析质膜中信号复合物的分子结构。
Cell Rep Methods. 2022 Feb 4;2(2):100165. doi: 10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28.
2
Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age.按代和年龄分组给予氨基酸混合物后运动活动和氧化应激相关因素的变化。
Int J Mol Sci. 2021 Sep 10;22(18):9822. doi: 10.3390/ijms22189822.
3
Mechanism of homodimeric cytokine receptor activation and dysregulation by oncogenic mutations.同源二聚体细胞因子受体的激活机制及致癌突变的失调。
Science. 2020 Feb 7;367(6478):643-652. doi: 10.1126/science.aaw3242.
4
Nanoscale Distribution of Nuclear Sites by Super-Resolved Image Cross-Correlation Spectroscopy.纳米级核位点的超分辨图像互相关光谱分布。
Biophys J. 2019 Dec 3;117(11):2054-2065. doi: 10.1016/j.bpj.2019.10.036. Epub 2019 Nov 2.
5
Mechanisms of inside-out signaling of the high-affinity IgG receptor FcγRI.高亲和力 IgG 受体 FcγRI 的内向外信号转导机制。
Sci Signal. 2018 Jul 24;11(540):eaaq0891. doi: 10.1126/scisignal.aaq0891.
6
Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials.多功能 DNA 纳米结构可将脂质膜穿孔并重塑为混合材料。
Nat Commun. 2018 Apr 18;9(1):1521. doi: 10.1038/s41467-018-02905-w.
7
Correlation analysis framework for localization-based superresolution microscopy.基于定位的超分辨率显微镜的相关分析框架。
Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3219-3224. doi: 10.1073/pnas.1711314115. Epub 2018 Mar 12.
8
Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments.配体诱导的 II 型白细胞介素-4 受体二聚体通过在质膜微区室内的快速再缔合得以维持。
Nat Commun. 2017 Jul 14;8:15976. doi: 10.1038/ncomms15976.
9
Decoupling global biases and local interactions between cell biological variables.解耦细胞生物学变量之间的全局偏差和局部相互作用。
Elife. 2017 Mar 13;6:e22323. doi: 10.7554/eLife.22323.
10
Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes.时空波动分析：分子过程未来纳米显微镜学的强大工具。
Biophys J. 2016 Aug 23;111(4):679-685. doi: 10.1016/j.bpj.2016.07.015.
本文引用的文献
1
Stoichiometry of molecular complexes at adhesions in living cells.活细胞中黏附处分子复合物的化学计量学。
Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2170-5. doi: 10.1073/pnas.0806036106. Epub 2009 Jan 23.
2
Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method.利用互相关光栅图像光谱法从激光扫描共聚焦图像序列中检测活细胞中的蛋白质复合物。
Biophys J. 2009 Jan;96(2):707-16. doi: 10.1016/j.bpj.2008.09.051.
3
Annexin A2 binding to endosomes and functions in endosomal transport are regulated by tyrosine 23 phosphorylation.膜联蛋白A2与内体的结合及在内体运输中的功能受酪氨酸23磷酸化的调控。
J Biol Chem. 2009 Jan 16;284(3):1604-11. doi: 10.1074/jbc.M806499200. Epub 2008 Nov 5.
4
Annexin A8 regulates late endosome organization and function.膜联蛋白A8调节晚期内体的组织和功能。
Mol Biol Cell. 2008 Dec;19(12):5267-78. doi: 10.1091/mbc.e08-04-0383. Epub 2008 Oct 15.
5
Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy.通过改进的空间图像互相关光谱法精确测量细胞中的蛋白质相互作用。
Mol Biosyst. 2008 Jun;4(6):672-85. doi: 10.1039/b719826d. Epub 2008 Apr 16.
6
Kinetics of morphogen gradient formation.形态发生素梯度形成的动力学
Science. 2007 Jan 26;315(5811):521-5. doi: 10.1126/science.1135774.
7
Particle image correlation spectroscopy (PICS): retrieving nanometer-scale correlations from high-density single-molecule position data.粒子图像相关光谱法（PICS）：从高密度单分子位置数据中获取纳米级相关性。
Biophys J. 2007 Jan 15;92(2):613-21. doi: 10.1529/biophysj.106.092577. Epub 2006 Nov 3.
8
The endocytic pathway and formation of the Wingless morphogen gradient.内吞途径与无翅形态发生素梯度的形成。
Development. 2006 Jan;133(2):307-17. doi: 10.1242/dev.02197. Epub 2005 Dec 14.
9
Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking and the diffusion model.由发动蛋白依赖性内吞作用形成的Dpp梯度：受体运输与扩散模型
Development. 2004 Oct;131(19):4843-56. doi: 10.1242/dev.01335.
10
Localization accuracy in single-molecule microscopy.单分子显微镜中的定位精度。
Biophys J. 2004 Feb;86(2):1185-200. doi: 10.1016/S0006-3495(04)74193-4.
Zotero 插件