Physics of Life Processes, Leiden Institute of Physics, Leiden University, Leiden, The Netherlands.
Biophys J. 2011 Apr 6;100(7):1810-8. doi: 10.1016/j.bpj.2010.12.3746.
A multitude of biological processes that involve multiple interaction partners are observed by two-color microscopy. Here we describe an analysis method for the robust quantification of correlation between signals in different color channels: particle image cross-correlation spectroscopy (PICCS). The method, which exploits the superior positional accuracy obtained in single-object and single-molecule microscopy, can extract the correlation fraction and length scale. We applied PICCS to correlation measurements in living tissues. The morphogen Decapentaplegic (Dpp) was imaged in wing imaginal disks of fruit fly larvae and we quantified what fraction of early endosomes contained Dpp.
双色显微镜可观察到涉及多个相互作用伙伴的多种生物过程。在此，我们描述了一种用于稳健定量分析不同颜色通道信号之间相关性的分析方法：粒子图像互相关光谱学（PICCS）。该方法利用单物体和单分子显微镜获得的优越位置精度，可以提取相关分数和长度尺度。我们将 PICCS 应用于活体组织中的相关测量。在果蝇幼虫的翅膀成虫盘（wing imaginal disks）中，我们对形态发生素 Decapentaplegic（Dpp）进行了成像，并定量了早期内体中包含 Dpp 的部分。
