新型半乳糖化聚阳离子脂质的合成及转染活性。

Synthesis and transfection activity of novel galactosylated polycationic lipid.

机构信息

Lomonosov Moscow State Academy of Fine Chemical Technology (MITHT), 86 Vernadskogo Ave., Moscow 119571, Russian Federation.

出版信息

Bioorg Med Chem Lett. 2011 May 15;21(10):2937-40. doi: 10.1016/j.bmcl.2011.03.056. Epub 2011 Mar 21.

DOI:10.1016/j.bmcl.2011.03.056

PMID:21463941

Abstract

In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mammalian cells, and was able both to compact plasmid DNA and to mediate cellular accumulation of various nucleic acids (ODN, pDNA and siRNA) exhibiting biological activity (transgene expression, gene silencing).

摘要

在这项研究中，我们合成了一种新的半乳糖基阳离子脂质，并研究了它的生物学活性。脂质的结构结合了 spermine 残基用于 DNA 压缩和半乳糖部分用于改善脂质体聚集行为。脂质对不同的哺乳动物细胞低毒，既能压缩质粒 DNA，又能介导各种核酸（ODN、pDNA 和 siRNA）的细胞积累，表现出生物活性（转基因表达、基因沉默）。