Department of Biology, Western Kentucky University, 1906 College Heights Blvd #110180, Bowling Green, KY 42101-1080, USA.
Plant Physiol Biochem. 2011 Jun;49(6):584-91. doi: 10.1016/j.plaphy.2011.02.017. Epub 2011 Feb 24.
Sunflower (Helianthus annuus L.) is a commercially important oilseed crop. Previous studies proved that this crop is a promising plant species for phytoextraction of excess soil phosphorus (P) because of its superior P accumulating characteristics. Suppression subtractive hybridization (SSH) strategy was employed to isolate and characterize genes that are induced in response to high P in this crop. SSH library was prepared using cDNA generated from plants treated with high P as the 'tester'. Based on the results of dot blot analysis, 360 positive cDNA clones were selected from the SSH library for sequencing. A total of 89 non-redundant expressed sequence tags (ESTs) were identified as high P-responsive genes and they were classified into 6 functional groups. Several genes involved in metabolism showed markedly preferential expression in the library. For further confirmation, thirteen of the representative ESTs were selected from all categories for RT-PCR analysis and the results showed up-regulation of these genes in response to high P-treatment. The gene expression data derived from this study suggested that several of the up-regulated genes identified under high P-treatment might be involved in P-accumulation and tolerance in this plant.
向日葵（Helianthus annuus L.）是一种具有商业重要性的油料作物。先前的研究证明，由于其卓越的磷积累特性，这种作物是一种很有前途的植物物种，可用于超量土壤磷（P）的植物提取。抑制性消减杂交（SSH）策略被用于分离和鉴定对高磷环境做出响应的基因。SSH 文库使用高磷处理的植物生成的 cDNA 作为“测试器”来制备。基于斑点印迹分析的结果，从 SSH 文库中选择了 360 个阳性 cDNA 克隆进行测序。总共鉴定出 89 个非冗余的表达序列标签（EST）为高磷响应基因，并将其分为 6 个功能组。参与代谢的几个基因在文库中表现出明显的优先表达。为了进一步验证，从所有类别中选择了 13 个代表性 EST 进行 RT-PCR 分析，结果表明这些基因在高磷处理下上调。本研究中的基因表达数据表明，在高磷处理下鉴定出的几个上调基因可能参与了该植物的磷积累和耐受力。
