Liang Shimin, Yang Fenghuan, Guan Wenjing, Wu Maosen, Chen Huamin, Tian Fang, Xu Yanli, He Chenyang
College of Life Sciences, Northeast Forest University, Haerbin 130012, China.
Wei Sheng Wu Xue Bao. 2011 Jan;51(1):29-34.
To better reveal the functions of key members involved in cyclic di-GMP signal metabolism pathways in the bacterial blight pathogen of rice Xanthomonas oryzae pv. oryzae (Xoo).
vieAxoo (PXO 04753), a gene putatively encoding the EAL domain proteins was investigated by gene deletion mutation using the marker exchange, complementation and phenotypic analysis.
The sequence of vieAxoo cloned from genomic DNA of the wild-type strain PXO99(A) was found to be highly conserved in plant-pathogenic Xanthomonas spp. VieAxoo was structurally featured with EAL and REC domains. No significant changes in virulence to rice, EPS production and flagellar motility were found in deltavieAxoo compared to PXO99(A), whereas remarkable changes in induction of hypersensitive responses (HR) in tobacco and biofilm formation were observed.
VieAxoo might function as an important reponse regulator in cyclic di-GMP signaling and regulation of bacterial induction of HR and biofilm formation of Xoo.
为了更好地揭示参与水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，Xoo）中环二鸟苷酸信号代谢途径的关键成员的功能。
使用标记交换、互补和表型分析，通过基因缺失突变研究了vieAxoo（PXO 04753），一个推测编码EAL结构域蛋白的基因。
从野生型菌株PXO99(A)的基因组DNA中克隆的vieAxoo序列在植物致病性黄单胞菌属中高度保守。VieAxoo具有EAL和REC结构域。与PXO99(A)相比，在deltavieAxoo中未发现对水稻的毒力、EPS产生和鞭毛运动有显著变化，而在烟草中观察到过敏反应（HR）诱导和生物膜形成有显著变化。
VieAxoo可能作为环二鸟苷酸信号传导以及Xoo细菌诱导HR和生物膜形成调控中的重要应答调节因子发挥作用。
